Clonal population expansion in an outbreak of Plasmodium falciparum on the northwest coast of Ecuador

Malaria Journal

Research

Claudia A. Vera-Arias¹  Fabián E. Sáenz¹  Gabriela Valenzuela¹  Eileen Vélez-Álvarez²  Naomi W. Lucchi³  Lindsay C. Morton³  Venkatachalam Udhayakumar³  Sheila Akinyi Okoth⁴  L. Enrique Castro⁵ 

[1] Centro de Investigación en Enfermedades Infecciosas y Crónicas, Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Ave. 12 de Octubre 1076 y Roca, Quito, Ecuador;Centro de Investigación en Enfermedades Infecciosas y Crónicas, Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Ave. 12 de Octubre 1076 y Roca, Quito, Ecuador;Universidad de las Fuerzas Armadas, Quito, Ecuador;Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, USA;Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, USA;Atlanta Research and Education Foundation, Decatur, GA, USA;Ministerio de Salud Pública, Guayaquil, Ecuador;

关键词: Plasmodium falciparum;    Malaria;    Ecuador;    Outbreak;    Microsatellite markers;   

DOI  :  10.1186/s12936-015-1019-2

 received in 2015-06-16, accepted in 2015-11-26,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundDetermining the source of malaria outbreaks in Ecuador and identifying remaining transmission foci will help in malaria elimination efforts. In this study, the genetic signatures of Plasmodium falciparum isolates, obtained from an outbreak that occurred in northwest Ecuador from 2012 to 2013, were characterized.MethodsMolecular investigation of the outbreak was performed using neutral microsatellites, drug resistance markers and pfhrp2 and pfhrp3 genotyping.ResultsA majority of parasite isolates (31/32) from this outbreak were of a single clonal type that matched a clonal lineage previously described on the northern coast of Peru and a historical isolate from Ecuador. All but one isolate carried a chloroquine-resistant pfcrt genotype and sulfadoxine- and pyrimethamine-sensitive pfdhps and pfdhfr genotypes. Pfmdr1 mutations were identified in codons 184 and 1042. In addition, most samples (97 %) showed presence of pfhrp2 gene.ConclusionsThis study indicates that parasites from a single clonal lineage largely contributed to this outbreak and this lineage was found to be genetically related to a lineage previously reported in the Peruvian coast and historical Ecuadorian parasites.

【 授权许可】

CC BY   
© Sáenz et al. 2015