Biocompatible coated magnetosome minerals with various organization and cellular interaction properties induce cytotoxicity towards RG-2 and GL-261 glioma cells in the presence of an alternating magnetic field

Journal of Nanobiotechnology

Research

François Guyot¹  Olivier Seksek²  Imène Chebbi³  Edouard Alphandéry⁴  Chalani Mandawala⁴  Raphael Le Fèvre⁵  Yasmina Hamdous⁶ 

[1] Institut de Minéralogie de Physique des Matériaux et de Cosmochimie, UMR 7590, CNRS, Université Pierre et Marie Curie, Muséum National d’Histoire Naturelle, Sorbonne Université, 4 Place Jussieu, 75005, Paris, France;Laboratoire d’Imagerie et Modélisation en Neurobiologie et Cancérologie (IMNC), Campus Universitaire, Bât. 440, 15 rue Georges Clemenceau, 91406, Orsay Cedex, France;Nanobacterie, 36 boulevard Flandrin, 75116, Paris, France;Nanobacterie, 36 boulevard Flandrin, 75116, Paris, France;Institut de Minéralogie de Physique des Matériaux et de Cosmochimie, UMR 7590, CNRS, Université Pierre et Marie Curie, Muséum National d’Histoire Naturelle, Sorbonne Université, 4 Place Jussieu, 75005, Paris, France;Nanobacterie, 36 boulevard Flandrin, 75116, Paris, France;Institut de Physique du Globe de Paris, Sorbonne Paris Cité, UMR 7154, CNRS, Université Paris Diderot, 1 rue Jussieu, 75005, Paris, France;Nanobacterie, 36 boulevard Flandrin, 75116, Paris, France;Laboratoire d’Imagerie et Modélisation en Neurobiologie et Cancérologie (IMNC), Campus Universitaire, Bât. 440, 15 rue Georges Clemenceau, 91406, Orsay Cedex, France;

关键词: Magnetosomes;    Magnetotactic bacteria;    Magnetosome minerals;    Magnetic hyperthermia;    Alternating magnetic field;    Cancer;    Oncology;    Nanomedicine;    Iron oxide;    nanoparticle;    nanotechnology;    nanooncology;   

DOI  :  10.1186/s12951-017-0293-2

 received in 2017-02-04, accepted in 2017-08-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBiologics magnetics nanoparticles, magnetosomes, attract attention because of their magnetic characteristics and potential applications. The aim of the present study was to develop and characterize novel magnetosomes, which were extracted from magnetotactic bacteria, purified to produce apyrogen magnetosome minerals, and then coated with Chitosan, Neridronate, or Polyethyleneimine. It yielded stable magnetosomes designated as M-Chi, M-Neri, and M-PEI, respectively. Nanoparticle biocompatibility was evaluated on mouse fibroblast cells (3T3), mouse glioblastoma cells (GL-261) and rat glioblastoma cells (RG-2). We also tested these nanoparticles for magnetic hyperthermia treatment of tumor in vitro on two tumor cell lines GL-261 and RG-2 under the application of an alternating magnetic field. Heating, efficacy and internalization properties were then evaluated.ResultsNanoparticles coated with chitosan, polyethyleneimine and neridronate are apyrogen, biocompatible and stable in aqueous suspension. The presence of a thin coating in M-Chi and M-PEI favors an arrangement in chains of the magnetosomes, similar to that observed in magnetosomes directly extracted from magnetotactic bacteria, while the thick matrix embedding M-Neri leads to structures with an average thickness of 3.5 µm2 per magnetosome mineral. In the presence of GL-261 cells and upon the application of an alternating magnetic field, M-PEI and M-Chi lead to the highest specific absorption rates of 120–125 W/gFe. Furthermore, while M-Chi lead to rather low rates of cellular internalization, M-PEI strongly associate to cells, a property modulated by the application of an alternating magnetic field.ConclusionsCoating of purified magnetosome minerals can therefore be chosen to control the interactions of nanoparticles with cells, organization of the minerals, as well as heating and cytotoxicity properties, which are important parameters to be considered in the design of a magnetic hyperthermia treatment of tumor.

【 授权许可】

CC BY   
© The Author(s) 2017