A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP–Chip

Biological Procedures Online

Methodology

Joanne L Attema¹  Holger Weishaupt¹ 

[1] Immunology Unit, Institute for Experimental Medical Science, BMC D14, Lund University, 221 84, Lund, Sweden;

关键词: Miniaturized chromatin immunoprecipitation assays;    Microarray technology;    Histone modifications;    Stem and progenitor cells;    Epigenetic regulation;    Lineage commitment;   

DOI  :  10.1007/s12575-010-9031-y

 received in 2010-03-25, accepted in 2010-04-21,  发布年份 2010

来源: Springer

【 摘 要 】

Dynamic chromatin structure is a fundamental property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during cellular differentiation and development. Analysis of chromatin structure using molecular biology and biochemical assays in rare somatic stem and progenitor cells is key for understanding these processes but poses a great challenge because of their reliance on millions of cells. Through the development of a miniaturized genome-scale chromatin immunoprecipitation method (miniChIP–chip), we have documented the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells and immediate progeny residing in murine bone marrow. In this report, we describe the miniChIP methodology that can be used for increasing an understanding of the epigenetic mechanisms underlying hematopoietic stem and progenitor cell function. Application of this method will reveal the contribution of dynamic chromatin structure in regulating the function of other somatic stem cell populations, and how this process becomes perturbed in pathological conditions.

【 授权许可】

CC BY   
© Weishaupt and Attema; licensee Springer 2010