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【摘要】

Dynamic chromatin structure is a fundamental property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during cellular differentiation and development. Analysis of chromatin structure using molecular biology and biochemical assays in rare somatic stem and progenitor cells is key for understanding these processes but poses a great challenge because of their reliance on millions of cells. Through the development of a miniaturized genome-scale chromatin immunoprecipitation method (miniChIP–chip), we have documented the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells and immediate progeny residing in murine bone marrow. In this report, we describe the miniChIP methodology that can be used for increasing an understanding of the epigenetic mechanisms underlying hematopoietic stem and progenitor cell function. Application of this method will reveal the contribution of dynamic chromatin structure in regulating the function of other somatic stem cell populations, and how this process becomes perturbed in pathological conditions.

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【授权许可】

2010 Weishaupt and Attema; licensee Springer

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Biological Procedures Online
A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP–Chip

Holger Weishaupt¹ Joanne L Attema¹
[1] Immunology Unit, Institute for Experimental Medical Science, BMC D14, Lund University, 221 84, Lund, Sweden
关键词: Lineage commitment; Epigenetic regulation; Stem and progenitor cells; Histone modifications; Microarray technology; Miniaturized chromatin immunoprecipitation assays;
Others : 794706 DOI : 10.1007/s12575-010-9031-y

received in 2010-03-25, accepted in 2010-04-21, 发布年份 2010
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【 摘 要 】

【 授权许可】

【 预 览 】

【 图 表 】

【 参考文献 】

【摘要】

【授权许可】

【预览】

【图表】

【参考文献】