Specific antibodies to Anopheles gSG6-P1 salivary peptide to assess early childhood exposure to malaria vector bites

Malaria Journal

Research

Rodolphe Ladekpo¹  Achille Massougbodji²  Célia Dechavanne³  Florence Migot-Nabias³  André Garcia³  David Courtin⁴  Gilles Cottrell⁵  Sylvie Cornélie⁶  Anne Poinsignon⁶  Manon Farce⁶  Franck Remoué⁷  Papa M Drame⁸ 

[1] Centre d’Etudes et de Recherche sur le Paludisme Associé à la Grossesse et à l’Enfant (CERPAGE), Cotonou, Benin;Centre d’Etudes et de Recherche sur le Paludisme Associé à la Grossesse et à l’Enfant (CERPAGE), Cotonou, Benin;Faculté des Sciences de la Santé, Université d’Abomey-Calavi, 01 BP 188, Cotonou, Benin;IRD UMR 216 Mère et enfant face aux infections tropicales, 75006, Paris, France;Faculté de Pharmacie, Université Paris Descartes, Sorbonne Paris Cité, 75006, Paris, France;IRD UMR 216 Mère et enfant face aux infections tropicales, 75006, Paris, France;Faculté de Pharmacie, Université Paris Descartes, Sorbonne Paris Cité, 75006, Paris, France;Centre d’Etudes et de Recherche sur le Paludisme Associé à la Grossesse et à l’Enfant (CERPAGE), Cotonou, Benin;IRD UMR 216 Mère et enfant face aux infections tropicales, 75006, Paris, France;Laboratoire de Mathématiques Appliquées, Université Paris Descartes, 75006, Paris, France;UMR MIVEGEC (IRD224-CNRS5290-Universités Montpellier 1 et 2), Institut de Recherche pour le Développement (IRD), BP64501, 34394, Montpellier, France;UMR MIVEGEC (IRD224-CNRS5290-Universités Montpellier 1 et 2), Institut de Recherche pour le Développement (IRD), BP64501, 34394, Montpellier, France;IRD-UMR MIVEGEC (IRD224-CNRS5290-Universités Montpellier 1 et 2), Centre de Recherche Entomologique de Cotonou (CREC), 01 BP 4414RP, Cotonou, Benin;UMR MIVEGEC (IRD224-CNRS5290-Universités Montpellier 1 et 2), Institut de Recherche pour le Développement (IRD), BP64501, 34394, Montpellier, France;IRD-UMR MIVEGEC (IRD224-CNRS5290-Universités Montpellier 1 et 2), Centre de Recherche Entomologique de Cotonou (CREC), 01 BP 4414RP, Cotonou, Benin;Laboratory of Parasitic Diseases, NIAID, NIH, 4 Center Dr, 20892-0425, Bethesda, MD, USA;

关键词: Malaria;    Salivary proteins;    Biomarker of exposure;    Anopheles;    Maternal IgG transfer;    Infants;    Africa;   

DOI  :  10.1186/s12936-015-0800-6

 received in 2015-04-22, accepted in 2015-07-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe estimates of risk of malaria in early childhood are imprecise given the current entomologic and parasitological tools. Thus, the utility of anti-Anopheles salivary gSG6-P1 peptide antibody responses in measuring exposure to Anopheles bites during early infancy has been assessed.MethodsAnti-gSG6-P1 IgG and IgM levels were evaluated in 133 infants (in Benin) at three (M3), six (M6), nine (M9) and 12 (M12) months of age. Specific IgG levels were also assessed in their respective umbilical cord blood (IUCB) and maternal blood (MPB).ResultsAt M3, 93.98 and 41.35% of infants had anti-gSG6-P1 IgG and IgM Ab, respectively. Specific median IgG and IgM levels gradually increased between M3 and M6 (p < 0.0001 and p < 0.001), M6–M9 (p < 0.0001 and p = 0.085) and M9–M12 (p = 0.002 and p = 0.03). These levels were positively associated with the Plasmodium falciparum infection intensity (p = 0.006 and 0.003), and inversely with the use of insecticide-treated bed nets (p = 0.003 and 0.3). Levels of specific IgG in the MPB were positively correlated to those in the IUCB (R = 0.73; p < 0.0001) and those at M3 (R = 0.34; p < 0.0001).ConclusionThe exposure level to Anopheles bites, and then the risk of malaria infection, can be evaluated in young infants by assessing anti-gSG6-P1 IgM and IgG responses before and after 6-months of age, respectively. This tool can be useful in epidemiological evaluation and surveillance of malaria risk during the first year of life.

【 授权许可】

CC BY   
© Drame et al. 2015