Predictive value of sperm deoxyribonucleic acid (DNA) fragmentation index in male infertility

Basic and Clinical Andrology

Research Article

Pramety Utami¹  Budi Wiweko¹ 

[1] Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jl. Diponegoro no. 71, Central Jakarta, Indonesia;

关键词: Cut-off point;    Male infertility;    Halosperm;    Semen analysis;    Sperm chromatin dispersion (SCD);    Sperm DNA fragmentation index;    valeur seuil;    infécondité masculine;    Halosperm;    analyse de sperme;    dispersion de la chromatine spermatozoïdaire (SCD);    indice de fragmentation de l’ADN spermatozoïdaire;   

DOI  :  10.1186/s12610-016-0046-3

 received in 2016-09-01, accepted in 2016-12-06,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundRecently, damage to the sperm DNA has been studied as it is associated with reduced fertilization rates, embryo quality, and pregnancy rates, also higher rates of spontaneous miscarriage.ObjectiveTo develop a diagnostic method in predicting male infertility.Material and MethodsThe design of this study is cross-sectional. Data were retrieved from medical records of Yasmin IVF Clinic Dr. Cipto Mangunkusumo General Hospital and Daya Medika Infertility Clinic from January to December 2015. Subjects were selected by consecutive sampling and divided into two groups: infertile and fertile. Sperm deoxyribonucleic acid fragmentation index (DFI) was determined by sperm chromatin dispersion (SCD) method using Halosperm® Kit.ResultsThere were 114 subjects (36 fertile and 78 infertile) selected into this study. We found no significant difference in the age between both of groups. The median value of sperm DFI in infertile group was significantly higher, 29.95 (26.6–34.3)%, compared to 19.90 (15.6–24.4)% of the fertile group, with p < 0.001. Area Under Curve (AUC) of sperm DFI, 0.862 (95% CI 0.783, 0.941), was higher than concentration (AUC 0.744; 95% CI 0.657, 0.831), motility (AUC 0.668; 95% CI 0.572, 0.765), and morphology (AUC 0.718; 95% CI 0.697, 0.864) of the semen analysis. At the cut-off point of 26.1%, the sperm DFI had sensitivity of 80.8% (95% CI; 70.0, 88.5), specificity of 86.1% (95% CI; 69.7, 94.8), positive predictive value (PPV) of 92.6% (95% CI; 83.0, 97.3), negative predictive value (NPV) of 67.4% (95% CI; 51.9, 80.0), positive likelihood ratio (PLR) of 12.6 (95% CI; 5.4, 29.4), and negative likelihood ratio (NLR) of 0.48 (95% CI 0.31, 0.75). Sperm DFI of ≥26.1% had prevalence ratio of 2.84 (95% CI 1.86, 4.33) for the occurrence of male infertility.ConclusionThere was significant difference between the median value of sperm DFI of infertile men and fertile men. Compared to semen analysis, sperm DFI at cut-off point of 26.1% has a higher diagnostic value (AUC).

【 授权许可】

CC BY   
© The Author(s). 2017