Molecular analysis demonstrates high prevalence of chloroquine resistance but no evidence of artemisinin resistance in Plasmodium falciparum in the Chittagong Hill Tracts of Bangladesh

Malaria Journal

Research

Fatema Tuj Johora¹  Kamala Thriemer²  Jutta Marfurt²  Benedikt Ley²  Sarah Auburn²  Ric N. Price³  Mohammad Enayet Hossain⁴  Mohammad Shafiul Alam⁴  Wasif A. Khan⁴  Maisha Khair Nima⁴ 

[1] Department of Zoology, University of Dhaka, Ramna, 1000, Dhaka, Bangladesh;Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia;Global and Tropical Health Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia;Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK;Infectious Diseases Division, International Centre for Diarrheal Diseases Research, Bangladesh Mohakhali, 1212, Dhaka, Bangladesh;

关键词: Plasmodium falciparum;    Antimalarial drug resistance;    Molecular markers;    Single nucleotide polymorphism;    pfcrt;    pfmdr1;    Artemisinin;    K13;    Bangladesh;   

DOI  :  10.1186/s12936-017-1995-5

 received in 2017-06-08, accepted in 2017-08-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundArtemisinin resistance is present in the Greater Mekong region and poses a significant threat for current anti-malarial treatment guidelines in Bangladesh. The aim of this molecular study was to assess the current status of drug resistance in the Chittagong Hill Tracts of Bangladesh near the Myanmar border.MethodsSamples were obtained from patients enrolled into a Clinical Trial (NCT02389374) conducted in Alikadam, Bandarban between August 2014 and January 2015. Plasmodium falciparum infections were confirmed by PCR and all P. falciparum positive isolates genotyped for the pfcrt K76T and pfmdr1 N86Y markers. The propeller region of the kelch 13 (k13) gene was sequenced from isolates from patients with delayed parasite clearance.ResultsIn total, 130 P. falciparum isolates were available for analysis. The pfcrt mutation K76T, associated with chloroquine resistance was found in 81.5% (106/130) of cases and the pfmdr1 mutation N86Y in 13.9% (18/130) cases. No single nucleotide polymorphisms were observed in the k13 propeller region.ConclusionThis study provides molecular evidence for the ongoing presence of chloroquine resistant P. falciparum in Bangladesh, but no evidence of mutations in the k13 propeller domain associated with artemisinin resistance. Monitoring for artemisinin susceptibility in Bangladesh is needed to ensure early detection and containment emerging anti-malarial resistance.

【 授权许可】

CC BY   
© The Author(s) 2017