Prevalence and clonal relationship of ESBL-producing Salmonella strains from humans and poultry in northeastern Algeria

BMC Veterinary Research

Research Article

Sana Hireche¹  Rachid Elgroud¹  Omar Bouaziz¹  Samia Djeffal²  Bakir Mamache³  Amir Agabou⁴  Kheira Rahal⁵  Sofiane Bakour⁶  Jean-Marc Rolain⁶  Selma Chabou⁶ 

[1] GSPA research Laboratory (Management of Animal Health and Productions), Institute of Veterinary Sciences, University Frères Mentouri Constantine 1, Constantine, Algeria;GSPA research Laboratory (Management of Animal Health and Productions), Institute of Veterinary Sciences, University Frères Mentouri Constantine 1, Constantine, Algeria;Institute of Veterinary and Agronomic Sciences, University Chadli Bendjedid, Eltarf, Algeria;Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France;Institute of Veterinary and Agronomic Sciences, University Hadj Lakhdar, Batna, Algeria;PADESCA Research Laboratory, Institute of Veterinary Sciences, University Frères Mentouri, Constantine, Algeria;Pasteur Institute, Medical Bacteriology Service, Algiers, Algeria;Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France;

关键词: Salmonella;    poultry;    human;    serotype;    antimicrobial resistance;    resistance genes;    clonality;   

DOI  :  10.1186/s12917-017-1050-3

 received in 2016-10-18, accepted in 2017-05-05,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe aims of this study were to investigate Salmonella contamination in broiler chicken farms and slaughterhouses, to assess the antibiotic resistance profile in avian and human Salmonella isolates, and to evaluate the relationship between avian and human Extended Spectrum β-Lactamase (ESBL)-producing isolates. Salmonella was screened in different sample matrices collected at thirty-two chicken farms and five slaughterhouses. The human isolates were recovered from clinical specimens at the University Teaching Hospital of Constantine (UTH). All suspected colonies were confirmed by MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time OF light) and serotyped. Susceptibility testing against 13 antibiotics including, amoxicillin/clavulanic acid, ticarcillin, cefoxitin, cefotaxime, aztreonam, imipenem, ertapenem, gentamicin, amikacin, ciprofloxacin, colistin, trimethoprim/sulfamethoxazole and fosfomycin, was performed using the disk diffusion method on Mueller-Hinton agar. ESBL-production was screened by the double-disk synergy test and confirmed by molecular characterization using PCR (polymerase chain reaction) amplification and sequencing of ESBL encoding genes. Clonality of the avian and human strains was performed using the Multi Locus Sequencing Typing method (MLST).ResultsForty-five isolated avian Salmonella strains and 37 human collected ones were studied. Five S. enterica serotypes were found in avian isolates (mainly Kentucky) and 9 from human ones (essentially Infantis). 51.11% and 26.6% of the avian isolates were resistant to ciprofloxacin and cefotaxime, respectively, whereas human isolates were less resistant to these antibiotics (13.5% to ciprofloxacin and 16.2% to cefotaxime). Eighteen (12 avian and 6 human) strains were found to produce ESBLs, which were identified as blaCTX-M-1 (n = 12), blaCTX-M-15 (n = 5) and blaTEM group (n = 8). Interestingly, seven of the ESBL-producing strains (5 avian and 2 human) were of the same ST (ST15) and clustered together, suggesting a common origin.ConclusionThe results of the combined phenotypic and genotypic analysis found in this study suggest a close relationship between human and avian strains and support the hypothesis that poultry production may play a role in the spread of multidrug-resistant Salmonella in the human community within the study region.

【 授权许可】

CC BY   
© The Author(s). 2017