Development of Recombinant Flagellar Antigens for Serological Detection of Salmonella enterica Serotypes Enteritidis, Hadar, Heidelberg, and Typhimurium in Poultry

Agriculture

Joseph Minicozzi³  Susan Sanchez¹  Margie D. Lee³  Peter S. Holt²  Charles L. Hofacre³ 

[1] Department of Infectious Diseases, The University of Georgia, Athens, GA 30602, USA; E-Mail:;Agricultural Research Service, United States Department of Agriculture, Athens, GA 30602, USA; E-Mail:;Department of Population Health, The University of Georgia, 953 College Station Rd., Athens, GA 30602, USA; E-Mails:

关键词: Salmonella;    ELISA;    poultry;    serotype;    Enteritidis;   

DOI  :  10.3390/agriculture3030381

来源: mdpi

【 摘 要 】

Accurate and fast detection of harmful Salmonella is a major concern of food safety. Common Salmonella serotypes responsible for human associated foodborne outbreaks are S. Enteritidis, S. Hadar, S. Heidelberg, and S. Typhimurium are also commonly isolated from poultry. Serology is commonly used to monitor disease in poultry, therefore application of Salmonella serotype-specific test will have added value in Salmonella surveillance or monitoring vaccine efficacy. Recombinant flagellins were purified to be used as antigens in an ELISA. In this study, an ELISA was developed for the serological detection of S. Enteritidis. Once optimized, 500 ng of purified recombinant S. Enteritidis flagellin and a 1:64 dilution were determined to be optimal for testing sera. A negative baseline cutoff was calculated to be an optical density (OD) of 0.35. All sera from birds with history of S. Enteritidis exposure tested positive and all sera from chickens with no exposure tested negative to this Salmonella serotype. Current ELISA for serological detection of Salmonella suffers from cross reactivity inherent in lipopolysaccharide (LPS) or whole cell antigen based serological tests. This new ELISA eliminates common cross reactivity by focusing specifically on the flagellins of the Salmonella serotypes common in poultry and associated with foodborne outbreaks.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.