Cytokine and chemokine profile of the innate and adaptive immune response of schistosoma haematobium and plasmodium falciparum single and co-infected school-aged children from an endemic area of Lambaréné, Gabon

Malaria Journal

Research

Jeannot F Zinsou¹  Roland F Kassa Kassa¹  Hermelijn Smits²  Maria Yazdanbakhsh³  Ulysse Ateba-Ngoa⁴  Ayola Akim Adegnika⁴  Benjamin Mordmüller⁵  Peter G Kremsner⁵  Marguerite Massinga-Loembe⁵ 

[1] Centre de Recherches Médicales de Lambaréné, BP: 118, Lambaréné, Gabon;Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2333, Leiden, ZA, The Netherlands;Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2333, Leiden, ZA, The Netherlands;Centre de Recherches Médicales de Lambaréné, BP: 118, Lambaréné, Gabon;Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2333, Leiden, ZA, The Netherlands;Institut für Tropenmedizin, Universität Tübingen, Wilhelmstraβe 27, D-72074, Tübingen, Germany;Centre de Recherches Médicales de Lambaréné, BP: 118, Lambaréné, Gabon;Institut für Tropenmedizin, Universität Tübingen, Wilhelmstraβe 27, D-72074, Tübingen, Germany;Centre de Recherches Médicales de Lambaréné, BP: 118, Lambaréné, Gabon;

关键词: Malaria;    Schistosomiasis;    Co-infection;    Innate immune response;    Adaptive immune response;    Principal component analysis;    Epidemiology;    Lambaréné;    School-aged children;   

DOI  :  10.1186/s12936-015-0608-4

 received in 2014-08-20, accepted in 2015-02-09,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundHelminths and malaria are among the most prevalent infectious diseases in the world. They both occur in tropical area where they often affect the same populations. There are studies suggesting an effect of helminths on malariometric indices. For example, malaria attacks as well as disease severity has been shown to be influenced by a concurrent chronic helminth infection. However, there are also studies that show no effect of concurrent helminth infections on malarial outcomes. To start addressing this issue, the effect of chronic Schistosoma haematobium infection on both the innate and adaptive immune response of Plasmodium falciparum-infected subjects was assessed in an area endemic for both these infections in Gabon.MethodSubjects infected with S. haematobium and or P. falciparum, as well as a control group with neither of these infections, were recruited. For innate immune response, heparinized blood was obtained and cultured for 24 hours with a panel of TLR ligands. For adaptive immune response, PBMC was isolated and stimulated with SEB for 72 hours. Cytokines and chemokines were measured in supernatants using a multiplex beads array immunoassay. Principal Component analysis was used to assess pattern of cytokine and chemokine responses representing the innate and adaptive components of the immune system.ResultsOverall it was observed that the presence of P. falciparum infection was marked by an increase in innate and adaptive immune responsiveness while S. haematobium infection was characterized by an increased chemokine profile, with at the same time, lower pro inflammatory markers. When the study subjects were split into single infected and co-infected groups no effect of S. haematobium on the immune response of P. falciparum infected subjects was observed, neither for the innate nor for the adaptive component of the immune response.ConclusionThis study provides original information on the cellular immune response of S. haematobium and/or P. falciparum in infected subjects. It rules out an effect of S. haematobium on the cytokine profile of subjects co-infected with P. falciparum.

【 授权许可】

Unknown   
© Ateba-Ngoa et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.