Anti-malarial IgG subclasses pattern and FcγRIIa (CD32) polymorphism among pregnancy-associated malaria in semi-immune Saudi women

Malaria Journal

Research

Amre Nasr¹  Gamal Allam²  Osama Hamid³  Abdelhamid Al-Ghamdi⁴ 

[1] Department of Microbiology, College of Medicine, Taif University, PO Box 888, Taif, Saudi Arabia;Department of Microbiology, Faculty of Science and Technology, Al-Neelain University, Khartoum, Sudan;Department of Basic Medical Sciences, College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia;Department of Microbiology, College of Medicine, Taif University, PO Box 888, Taif, Saudi Arabia;Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt;Department of Public Health, Jazan Health Affairs District MoH, Jazan, Saudi Arabia;Department of Surgery, College of Medicine, Taif University, Taif, Saudi Arabia;

关键词: Pregnant;    Asymptomatic;    Malaria;    IgG;    Subclasses;    FcγRIIa;    Polymorphism;    Saudi Arabia;   

DOI  :  10.1186/1475-2875-12-110

 received in 2012-11-21, accepted in 2013-03-16,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPregnant women remain are at an increased risk of malaria with primigravidae being at the highest risk. Genetic polymorphism of the Fc receptor IIa for immunologlobulin (Ig) G (FcγRIIa) determines IgG subclass binding. Protection against pregnancy-associated malaria (PAM) is associated with the production of IgG specific for apical membrane antigen-1 (AMA-1). The present study was undertaken to examine the relationship between specific IgG/IgG subclasses and malaria infection. The second aim of the study is to examine the association between FcγRIIa R/H131 polymorphism in correlation with specific anti-malarial IgG antibodies of AMA-1 distribution and asymptomatic malaria infection among Saudi women living in the southern part of Saudi Arabia.MethodsOne hundred and twenty pregnant women living in an area of meso-endemic Plasmodium falciparum malaria infection were consecutively enrolled onto the study. These pregnant women were asymptomatic and attending routine antenatal clinics. The levels of plasma antibodies (IgG and subclasses AMA-1) were measured using indirect enzyme-linked immunosorbent assays (ELISA). Genotyping of FcγRIIa-R/H131 dimorphism was performed using gene-specific polymerase chain reaction (PCR) amplification with allele-specific restriction enzyme digestion (Bst U1) of the PCR product.ResultsA total of sixty-two (52%) pregnant women was diagnosed with asymptomatic malarial infection (ASM) compared with 58 (48%) malaria free controls (MFC). In the ASM group, there were high levels of anti-malarial IgG1 and IgG3, when compared to MFC (P value <0.001, respectively). The FcγRIIa-R/R131 genotype and R131 were found to be statistically significantly more prevalent in the ASM group when compared to the MFC group [55% for ASM versus 12% for MFC, odds ratio (OR) 5.62, 95% confidence interval (CI)= (2.03- 15.58), P value= 0.001]. However, the H/H131 genotype showed statistically significant association with MFC [14% for ASM versus 50% for MFC, OR(0.36), 95% CI= (0.14- 0.95), P value= 0.03].ConclusionsThe study revealed that the ASM patients had higher anti-malarial IgG and IgG subclasses antibody levels when compared to the MFC. The FcγRIIa-R/R131 genotype and R131 allele were found to be statistically prevalent in the ASM when compared to the MFC group. The individuals carrying H/H131 were consistently associated with higher levels of anti-malarial IgG subclasses.

【 授权许可】

Unknown   
© Nasr et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.