Significance of CO2 donor on the production of succinic acid by Actinobacillus succinogenes ATCC 55618

Microbial Cell Factories

Research

Wei Zou¹  Hong-Mei Li¹  Li-Wen Zhu¹  Ya-Jie Tang² 

[1] Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, 430068, Wuhan, China;Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, 430068, Wuhan, China;Laboratory of Synthetic Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 201203, Shanghai, China;National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100080, Beijing, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China;

关键词: Succinic Acid;    Fermentation Broth;    MgCO3;    Succinic Acid Production;    Acetic Acid Production;   

DOI  :  10.1186/1475-2859-10-87

 received in 2011-06-30, accepted in 2011-10-31,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSuccinic acid is a building-block chemical which could be used as the precursor of many industrial products. The dissolved CO2 concentration in the fermentation broth could strongly regulate the metabolic flux of carbon and the activity of phosphoenolpyruvate (PEP) carboxykinase, which are the important committed steps for the biosynthesis of succinic acid by Actinobacillus succinogenes. Previous reports showed that succinic acid production could be promoted by regulating the supply of CO2 donor in the fermentation broth. Therefore, the effects of dissolved CO2 concentration and MgCO3 on the fermentation process should be investigated. In this article, we studied the impacts of gaseous CO2 partial pressure, dissolved CO2 concentration, and the addition amount of MgCO3 on succinic acid production by Actinobacillus succinogenes ATCC 55618. We also demonstrated that gaseous CO2 could be removed when MgCO3 was fully supplied.ResultsAn effective CO2 quantitative mathematical model was developed to calculate the dissolved CO2 concentration in the fermentation broth. The highest succinic acid production of 61.92 g/L was obtained at 159.22 mM dissolved CO2 concentration, which was supplied by 40 g/L MgCO3 at the CO2 partial pressure of 101.33 kPa. When MgCO3 was used as the only CO2 donor, a maximal succinic acid production of 56.1 g/L was obtained, which was just decreased by 7.03% compared with that obtained under the supply of gaseous CO2 and MgCO3.ConclusionsBesides the high dissolved CO2 concentration, the excessive addition of MgCO3 was beneficial to promote the succinic acid synthesis. This was the first report investigating the replaceable of gaseous CO2 in the fermentation of succinic acid. The results obtained in this study may be useful for reducing the cost of succinic acid fermentation process.

【 授权许可】

Unknown   
© Zou et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.