Synthesis and characterization of crosslinked polyisothiouronium methylstyrene nanoparticles of narrow size distribution for antibacterial and antibiofilm applications

Journal of Nanobiotechnology

Research

Enav Corem-Salkmon¹  Shlomo Margel¹  Chen Gelber¹  Sarit Cohen¹  Ehud Banin²  Michal Natan² 

[1] Department of Chemistry, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, 52900, Ramat Gan, Israel;The Mina and Everard Goodman Faculty of Life Sciences, The Institute for Advanced Materials and Nanotechnology, Bar-Ilan University, 52900, Ramat Gan, Israel;

关键词: Isothioronium methylstyrene;    Dispersion polymerization;    Nanoparticles;    Antibacterial;    Biofilm;   

DOI  :  10.1186/s12951-016-0208-7

 received in 2016-02-14, accepted in 2016-06-20,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundIsothiouronium salts are well known in their variety of antimicrobials activities. The use of polymeric biocides, polymers with antimicrobial activities, is expected to enhance the efficacy of some existing antimicrobial agents, thus minimizing the environmental problems accompanying conventional antimicrobials.MethodsThe current manuscript describes the synthesis and characterization of crosslinked polyisothiouronium methylstyrene (PITMS) nanoparticles (NPs) of narrow size distribution by dispersion co-polymerization of the monomer isothiouronium methylstyrene with the crosslinking monomer ethylene glycol dimetacrylate.Results and discussionThe effect of total monomer, crosslinker and initiator concentrations on the size and size distribution of the formed NPs was also elucidated. The bactericidal activity of PITMS NPs of 67 ± 8 nm diameter was illustrated for 4 bacterial pathogens: Listeria innocua, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In order to demonstrate the potential of these PITMS NPs as inhibitor of biofilm formation, polyethylene terephthalate (PET) films were thin-coated with the PITMS NPs. The formed PET/PITMS films reduced the viability of the biofilm of Listeria by 2 orders of magnitude, making the coatings excellent candidates for further development of non-fouling surfaces. In addition, PITMS NP coatings were found to be non-toxic in HaCaT cells.ConclusionsThe high antibacterial activity and effective inhibition of bacterial adsorption indicate the potential of these nanoparticles for development of new types of antibacterial and antibiofilm additives.

【 授权许可】

CC BY   
© The Author(s) 2016