CePP2C19 confers tolerance to drought by regulating the ABA sensitivity in Cyperus esculentus

BMC Plant Biology

Research

Yifei Wang¹  Yuanyuan Dong¹  Fawei Wang¹  Hengshuo Ge¹  Yijin Wang¹  Weican Liu¹  Xiaowei Li¹  Jia Li¹  Xinyi Liu¹  Nan Wang¹  Naveed Ahmad² 

[1] College of Life Sciences, Jilin Agricultural University, 130118, Changchun, China;Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, China;

关键词: Protein phosphatase 2 C (PP2Cs);    ABA signaling;    Drought stress;    Arabidopsis;    Tiger nut;   

DOI  :  10.1186/s12870-023-04522-2

 received in 2023-04-04, accepted in 2023-10-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundTiger nut (Cyperus esculentus) is widely known as an additional source of food, oil and feed worldwide. The agricultural production of tiger nut has been greatly hindered by drought stress, reducing both yield and quality. Protein phosphatase 2 C (PP2Cs) plays an important role in plant responses to drought stress however, the molecular mechanism of PP2Cs in tiger nuts still unclear.ResultsIn this study, we identified a putative group A PP2C-encoding gene (CePP2C19) from tiger nut using transcriptome analysis, which is highly induced by drought stress. The transient expression assay suggested that CePP2C19 was localized to nucleus. Furthermore, the interaction between CePP2C19 and CePYR1, a coreceptor for ABA signaling, was first detected using a yeast two-hybrid assay and then verified using a bimolecular fluorescence complementation (BiFC) analysis. In addition, the transgenic Arabidopsis lines overexpressing CePP2C19 exhibited extreme tolerance to ABA and mannitol stresses during seed germination and root growth. At the mature stage, overexpression of CePP2C19 resulted in a higher tolerance to drought stress in transgenic Arabidopsis, as confirmed by a visible phenotype and several physiological parameters. Noticeably, the silencing of CePP2C19 by virus-induced gene silencing (VIGS) showed obvious reduction in drought tolerance in tiger nut plants.ConclusionsThe CePP2C19 emerges as a pivotal gene involved in the ABA signaling pathway, which likely reduce ABA sensitivity and thus enhances drought tolerance in Cyperus esculentus.

【 授权许可】

CC BY   
© The Author(s) 2023

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