Prevalence of class 1 and 2 integrons in multi-drug resistant Escherichia coli isolated from aquaculture water in Chaharmahal Va Bakhtiari province, Iran

Annals of Clinical Microbiology and Antimicrobials

Research

Elahe Tajbakhsh¹  Ifeoma Chinyere Ugwu²  Reza Ranjbar³  Faham Khamesipour⁴ 

[1] Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran;Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria;Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran;

关键词: Antimicrobial resistance;    Aquaculture;    E. coli;    Integrons;    Iran;   

DOI  :  10.1186/s12941-015-0096-y

 received in 2014-11-20, accepted in 2015-06-25,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIntegrons play important role in the spread and maintenance of antimicrobial resistance among strains of Escherichia coli (E. coli) and other species of Enterobacteriaceae. This study investigated the prevalence of class 1 and 2 integrons among E. coli strains isolated from aquaculture water of fish fields in Iran.MethodsOne hundred and fifty water samples from different geographical regions in Chaharmahal Va Bakhtiari province were examined over a 2 months period. Isolation was through culture and biochemical tests. Integrons were identified through polymerase chain reaction (PCR) using oligonucleotide primers specific for class 1 and 2 integrons. Antimicrobial susceptibility testing was carried out using disc diffusion methods.ResultsEighteen percent of the water samples were positive for E. coli. All the strains were multi-drug resistant; 100% to ciprofloxacin, chloramphenicol, gentamycin, ampicillin and tetracycline and least resistant to imipenem (7.2%). Ten (50%) of the most resistant strains were positive for class 1 (40%) and class 2 (10%).ConclusionsEscherichia coli in aquaculture in Iran carried integrons class 1 and 2 which could be of public health concern since they could play a role in the spread and maintenance of antimicrobial resistance among bacterial population in the region and should be constantly monitored.

【 授权许可】

CC BY   
© Tajbakhsh et al. 2015