Effects of aromatic compounds on the production of bacterial nanocellulose by Gluconacetobacter xylinus

Microbial Cell Factories

Research

Leif J Jönsson¹  Feng Hong²  Xiang Guo²  Shuo Zhang³  Sandra Winestrand⁴  Lin Chen⁵ 

[1] China-Sweden Associated Research Laboratory in Industrial Biotechnology, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 201620, Shanghai, China;Department of Chemistry, Umeå University, SE-901 87, Umeå, Sweden;China-Sweden Associated Research Laboratory in Industrial Biotechnology, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 201620, Shanghai, China;Group of Microbiological Engineering and Industrial Biotechnology, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 201620, Shanghai, China;China-Sweden Associated Research Laboratory in Industrial Biotechnology, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 201620, Shanghai, China;Group of Microbiological Engineering and Industrial Biotechnology, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 201620, Shanghai, China;School of Environmental Science & Engineering, Donghua University, 201620, Shanghai, China;Department of Chemistry, Umeå University, SE-901 87, Umeå, Sweden;Group of Microbiological Engineering and Industrial Biotechnology, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 201620, Shanghai, China;

关键词: Gluconacetobacter xylinus;    Phenolic compound;    Bacterial cellulose;    Inhibitor;   

DOI  :  10.1186/1475-2859-13-62

 received in 2013-12-31, accepted in 2014-04-21,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundBacterial cellulose (BC) is a polymeric nanostructured fibrillar network produced by certain microorganisms, principally Gluconacetobacter xylinus. BC has a great potential of application in many fields. Lignocellulosic biomass has been investigated as a cost-effective feedstock for BC production through pretreatment and hydrolysis. It is well known that detoxification of lignocellulosic hydrolysates may be required to achieve efficient production of BC. Recent results suggest that phenolic compounds contribute to the inhibition of G. xylinus. However, very little is known about the effect on G. xylinus of specific lignocellulose-derived inhibitors. In this study, the inhibitory effects of four phenolic model compounds (coniferyl aldehyde, ferulic acid, vanillin and 4-hydroxybenzoic acid) on the growth of G. xylinus, the pH of the culture medium, and the production of BC were investigated in detail. The stability of the phenolics in the bacterial cultures was investigated and the main bioconversion products were identified and quantified.ResultsConiferyl aldehyde was the most potent inhibitor, followed by vanillin, ferulic acid, and 4-hydroxybenzoic acid. There was no BC produced even with coniferyl aldehyde concentrations as low as 2 mM. Vanillin displayed a negative effect on the bacteria and when the vanillin concentration was raised to 2.5 mM the volumetric yield of BC decreased to ~40% of that obtained in control medium without inhibitors. The phenolic acids, ferulic acid and 4-hydroxybenzoic acid, showed almost no toxic effects when less than 2.5 mM. The bacterial cultures oxidized coniferyl aldehyde to ferulic acid with a yield of up to 81%. Vanillin was reduced to vanillyl alcohol with a yield of up to 80%.ConclusionsThis is the first investigation of the effect of specific phenolics on the production of BC by G. xylinus, and is also the first demonstration of the ability of G. xylinus to convert phenolic compounds. This study gives a better understanding of how phenolic compounds and G. xylinus cultures are affected by each other. Investigations in this area are useful for elucidating the mechanism behind inhibition of G. xylinus in lignocellulosic hydrolysates and for understanding how production of BC using lignocellulosic feedstocks can be performed in an efficient way.

【 授权许可】

Unknown   
© Zhang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.