Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers

Reproductive Biology and Endocrinology

Research

Usanee Sanmee¹  Sudarat Samchimchom¹  Chanchai Chailert¹  Waraporn Piromlertamorn¹  Teraporn Vutyavanich¹ 

[1] Department of Obstetric and Gynecology, Division of Reproductive Medicine,Faculty of Medicine, Chiang Mai University, 50200, Chiang Mai, Thailand;

关键词: Assisted hatching;    Laser;    Differential staining;    Zona drilling;    Zona pellucida;   

DOI  :  10.1186/1477-7827-11-21

 received in 2012-11-08, accepted in 2013-03-13,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundIt is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers.MethodsIn experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the zona pellucida with an infrared laser beam. In the second experiment, 0, 1 and 2 openings of 20 microns were studied. In the third experiment, a full-thickness opening of 20 microns or quarter-thinning of the zonal circumference to a depth of 90% was compared with non-AH controls.ResultsNo difference in blastocyst formation was found in laser-treated groups and in the controls. In experiment 1, the rate of completely hatched blastocysts was significantly lower than the controls. In experiment 2 when the size of the opening was increased, blastocysts completely hatched at a significantly higher rate than that in the controls. In experiment 3, the rate of completely hatched blastocysts was the highest in the full-thickness group. Cell numbers in completely hatched blastocysts from both AH groups were significantly fewer than those in the controls.ConclusionsFull-thickness opening resulted in a higher rate of completely hatched blastocysts than quarter zonal-thinning and controls, but the cell numbers were significantly decreased.

【 授权许可】

Unknown   
© Chailert et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.