Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers

Reproductive Biology and Endocrinology

Teraporn Vutyavanich¹  Sudarat Samchimchom¹  Waraporn Piromlertamorn¹  Usanee Sanmee¹  Chanchai Chailert¹ 

[1] Division of Reproductive Medicine, Department of Obstetric and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand

关键词: Zona pellucida;    Zona drilling;    Differential staining;    Laser;    Assisted hatching;   

Others  :  812474
DOI  :  10.1186/1477-7827-11-21

 received in 2012-11-08, accepted in 2013-03-13,  发布年份 2013

【 摘 要 】

Background

It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers.

Methods

In experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the zona pellucida with an infrared laser beam. In the second experiment, 0, 1 and 2 openings of 20 microns were studied. In the third experiment, a full-thickness opening of 20 microns or quarter-thinning of the zonal circumference to a depth of 90% was compared with non-AH controls.

Results

No difference in blastocyst formation was found in laser-treated groups and in the controls. In experiment 1, the rate of completely hatched blastocysts was significantly lower than the controls. In experiment 2 when the size of the opening was increased, blastocysts completely hatched at a significantly higher rate than that in the controls. In experiment 3, the rate of completely hatched blastocysts was the highest in the full-thickness group. Cell numbers in completely hatched blastocysts from both AH groups were significantly fewer than those in the controls.

Conclusions

Full-thickness opening resulted in a higher rate of completely hatched blastocysts than quarter zonal-thinning and controls, but the cell numbers were significantly decreased.

【 授权许可】

   
2013 Chailert et al.; licensee BioMed Central Ltd.

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