Polymeric micelles with dual thermal and reactive oxygen species (ROS)-responsiveness for inflammatory cancer cell delivery

Journal of Nanobiotechnology

Research

Yanfang Wang¹  Nicola Tirelli²  Qing Tang³  Meiqiong Tang³  Yun He³  Qiang Zheng³  Xiaohong Yang³  Ping Hu³  Zhanlong Wang³ 

[1] First Affiliated Hospital of the Medical College, Shihezi University, 832008, Xinjiang, People’s Republic of China;NorthWest Centre of Advanced Drug Delivery (NoWCADD), School of Pharmacy, and Centre for Tissue Injury and Repair, Institute of Inflammation and Repair, University of Manchester, Oxford Road, M13 9PT, Manchester, UK;School of Pharmaceutical Sciences, Chongqing University, 55 South Daxuecheng Road, 401331, Chongqing, China;

关键词: Nanomedicine;    Polymeric micelle;    ROS-responsive;    Cellular uptake;   

DOI  :  10.1186/s12951-017-0275-4

 received in 2016-12-19, accepted in 2017-05-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe object of this study was to develop a thermally and reactive oxygen species-responsive nanocarrier system for cancer therapy.ResultsPPS-PNIPAm block copolymer was designed and synthesised using a combination of living anionic ring-opening polymerization and atom transfer radical polymerization. The synthesized polymer formed micellar aggregates in water and demonstrated dual responsiveness towards temperature and oxidants. Using doxorubicin (DOX) as a model drug, encapsulation and in vitro release of the drug molecules in PPS-PNIPAm nanocarriers confirmed the responsive release properties of such system. Cell uptake of the DOX loaded micelles was investigated with human breast cancer cell line (MCF-7). The results showed Dox-loaded micelles were able to be taken by the cells and mainly reside in the cytoplasma. In the stimulated cells with an elevated level of ROS, more released DOX was observed around the nuclei. In the cytotoxicity experiments, the Dox-loaded micelles demonstrated comparable efficacy to free DOX at higher concentrations, especially on ROS stimulated cells.ConclusionsThese results demonstrated that PPS-PNIPAm nanocarriers possess the capability to respond two typical stimuli in inflammatory cells: temperature and oxidants and can be used in anticancer drug delivery.

【 授权许可】

CC BY   
© The Author(s) 2017