Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia

Proteome Science

Research

Pavel Májek¹  Zuzana Reicheltová¹  Jaroslav Čermák¹  Jiří Suttnar¹  Jan E Dyr¹ 

[1] Department of Biochemistry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic;

关键词: myelodysplastic syndrome;    refractory cytopenia;    dysplasia;    proteome;   

DOI  :  10.1186/1477-5956-9-64

 received in 2011-05-23, accepted in 2011-10-05,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundRefractory cytopenia with multilineage dysplasia (RCMD) is a subgroup of myelodysplastic syndrome (MDS), which belongs to oncohematological diseases, occurring particularly in elderly patients, and represents a heterogeneous group of bone marrow diseases. The goal of this study was to look for plasma proteins that changed quantitatively or qualitatively in RCMD patients.ResultsA total of 46 plasma samples were depleted, proteins were separated by 2D SDS-PAGE (pI 4-7), and proteomes were compared using Progenesis SameSpots statistical software. Proteins were identified by nanoLC-MS/MS. Sixty-one unique, significantly (p < 0.05, ANOVA) different spots were found; proteins in 59 spots were successfully identified and corresponded to 57 different proteins. Protein fragmentation was observed in several proteins: complement C4-A, complement C4-B, inter-alpha-trypsin inhibitor heavy chain H4, and endorepellin.ConclusionsThis study describes proteins, which change quantitatively or qualitatively in RCMD patients, and represents the first report on significant alterations in C4-A and C4-B complement proteins and ITIH4 fragments in patients with MDS-RCMD.

【 授权许可】

CC BY   
© Májek et al; licensee BioMed Central Ltd. 2011