Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia

Proteome Science

Jan E Dyr¹  Jaroslav Čermák¹  Jiří Suttnar¹  Zuzana Reicheltová¹  Pavel Májek¹ 

[1] Department of Biochemistry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic

关键词: proteome;    dysplasia;    refractory cytopenia;    myelodysplastic syndrome;   

Others  :  820192
DOI  :  10.1186/1477-5956-9-64

 received in 2011-05-23, accepted in 2011-10-05,  发布年份 2011

【 摘 要 】

Background

Refractory cytopenia with multilineage dysplasia (RCMD) is a subgroup of myelodysplastic syndrome (MDS), which belongs to oncohematological diseases, occurring particularly in elderly patients, and represents a heterogeneous group of bone marrow diseases. The goal of this study was to look for plasma proteins that changed quantitatively or qualitatively in RCMD patients.

Results

A total of 46 plasma samples were depleted, proteins were separated by 2D SDS-PAGE (pI 4-7), and proteomes were compared using Progenesis SameSpots statistical software. Proteins were identified by nanoLC-MS/MS. Sixty-one unique, significantly (p < 0.05, ANOVA) different spots were found; proteins in 59 spots were successfully identified and corresponded to 57 different proteins. Protein fragmentation was observed in several proteins: complement C4-A, complement C4-B, inter-alpha-trypsin inhibitor heavy chain H4, and endorepellin.

Conclusions

This study describes proteins, which change quantitatively or qualitatively in RCMD patients, and represents the first report on significant alterations in C4-A and C4-B complement proteins and ITIH4 fragments in patients with MDS-RCMD.

【 授权许可】

   
2011 Májek et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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