In vitro physiological and antibacterial characterization of ZnO nanoparticle composites in simulated porcine gastric and enteric fluids

BMC Veterinary Research

Research Article

Marina S.R. Barreto¹  Cristina T. Andrade¹  Eduardo M. Del Aguila²  Vânia M. Flosi Paschoalin²  Luiz Cláudio R. P. da Silva³  Lúcio M. Cabral³ 

[1] Instituto de Macromoléculas Professora Eloisa Mano, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco J, 21941-598, Rio de Janeiro, RJ, Brazil;Instituto de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira, Ramos 149, Bloco A, sala 545, 21941-909, Rio de Janeiro, Brazil;Universidade Federal do Rio de Janeiro, Faculdade de Farmácia, Av. Carlos Chagas Filho, 373, CCS, Bloco L, sala 24, 21941-902, Rio de Janeiro, RJ, Brazil;

关键词: Chitosan/alginate coatings;    ZnO nanoparticles;    Biological properties;    Piglets;   

DOI  :  10.1186/s12917-017-1101-9

 received in 2016-08-30, accepted in 2017-06-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundDiarrhea in piglets is one of the main causes of animal death after weaning; zinc oxide (ZnO) has been used in high doses for the control of this sickness. The aim of this study was to determine the physicochemical properties of ZnO nanoparticles synthesized and immobilized on a chitosan/alginate (CH/SA) complex and investigate the antimicrobial activity and in vitro release profile of zinc (Zn2+) from these new compounds. The ZnO nanoparticles composites were prepared and combined with CH/SA or CH/SA and sodium tripolyphosphate (TPP). The structure and morphology of the composites were analyzed by characterization methods such as X-ray diffraction, FTIR spectroscopy, thermogravimetric analysis, atomic absorption spectrophotometry and scanning electron microscopy.ResultsThe crystallite size of ZnO nano was 17 nm and the novel ZnO composites were effective in protecting ZnO in simulated gastric fluid, where Zn2+ reached a concentration six-fold higher than the levels obtained with the unprotected commercial-zinc oxide. In addition, the novel composites suggest effective antimicrobial activity against Escherichia coli and Staphylococcus aureus.ConclusionsThe results described herein suggest that the novel nano composites may work as an alternative product for pig feeding as verified by the in vitro assays, and may also contribute to lower the zinc released in the environment by fecal excretion in animals waste.

【 授权许可】

CC BY   
© The Author(s). 2017