Design and development of alginate: Poly-l-lysine scaffolds by 3D bio printing and studying their mechanical, structural and cell viability properties.

2nd International conference on Advances in Mechanical Engineering

Datta, S.^1 ; Barua, R.^1 ; Sarkar, R.^1 ; Barui, A.^1 ; Chowdhury, A. Roy^2 ; Datta, P.^1

Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, Howrah

711103, India^1

Department of Aerospace Engineering and Applied Mechanics, Indian Institute of Engineering Science and Technology, Shibpur, Howrah

711103, India^2

关键词: 3-D printing;    Adipose tissue engineering;    Biological properties;    Biological scaffolds;    Design and Development;    Functional tissue;    Printed structures;    Strength and stiffness;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/402/1/012113/pdf
DOI  :  10.1088/1757-899X/402/1/012113

来源: IOP

【 摘 要 】

3D bio-printing is a very rapid and vigorous process which is used for fabricating the in-vitro biological functional tissues. For printing an effective scaffold, it is very imperative; the printed structure must have adequate strength and stiffness for keeping structural veracity. Alginates are such a biomaterial which can be used as a bio-ink for printing the 3D biological scaffolds. Also the bio-ink gel must not be very strong because more viscous results huge shear force for extruding the bio-ink from the nozzle which may results in cell fracture and death. Alginate is such a biocompatible and healthy material for cell. There are many applications of alginate bio-ink in bone, adipose tissue engineering and in vascular field. In this paper we have investigated the biological properties of alginate bio-ink based 3D bio-printed scaffold by using different percentage of honey mixing with same percentage of alginate. Then we characterized the bio-printed scaffolds with the help of UTM, Surfacetensiometer, MTT and SEM. We have studied the mechanical, physic-chemical and the cell viability properties of alginate 5%, alginate 5%:poly-l-lysine 1.5% and alginate 5%:poly-l-lysine 2.5% cross-linked with 1 molar CaCl2 with printing speed of 50mm/s.