Increasing the source/sink ratio in Vitis vinifera (cv Sangiovese) induces extensive transcriptome reprogramming and modifies berry ripening

BMC Genomics

Research Article

Giovanni Battista Tornielli¹  Silvia Dal Santo¹  Mario Pezzotti¹  Sara Zenoni¹  Gianluca Allegro²  Gabriele Valentini²  Ilaria Filippetti²  Chiara Pastore²  Cesare Intrieri² 

[1] Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy;Department of Fruit Tree and Woody Plant Science, University of Bologna, Viale Fanin, 46, 40126, Bologna, Italy;

关键词: Anthocyanin Content;    Anthocyanin Biosynthesis;    Grape Berry;    Cinnamyl Alcohol Dehydrogenase;    Sucrose Transporter;   

DOI  :  10.1186/1471-2164-12-631

 received in 2011-08-24, accepted in 2011-12-23,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCluster thinning is an agronomic practice in which a proportion of berry clusters are removed from the vine to increase the source/sink ratio and improve the quality of the remaining berries. Until now no transcriptomic data have been reported describing the mechanisms that underlie the agronomic and biochemical effects of thinning.ResultsWe profiled the transcriptome of Vitis vinifera cv. Sangiovese berries before and after thinning at veraison using a genome-wide microarray representing all grapevine genes listed in the latest V1 gene prediction. Thinning increased the source/sink ratio from 0.6 to 1.2 m2 leaf area per kg of berries and boosted the sugar and anthocyanin content at harvest. Extensive transcriptome remodeling was observed in thinned vines 2 weeks after thinning and at ripening. This included the enhanced modulation of genes that are normally regulated during berry development and the induction of a large set of genes that are not usually expressed.ConclusionCluster thinning has a profound effect on several important cellular processes and metabolic pathways including carbohydrate metabolism and the synthesis and transport of secondary products. The integrated agronomic, biochemical and transcriptomic data revealed that the positive impact of cluster thinning on final berry composition reflects a much more complex outcome than simply enhancing the normal ripening process.

【 授权许可】

Unknown   
© Pastore et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.