Genetic mechanisms underlying the methylation level of anthocyanins in grape (Vitis viniferaL.)

BMC Plant Biology

Research Article

Clotilde Verriès¹  Agnès Ageorges¹  Patrice This²  Alexandre Fournier-Level³  Philippe Hugueney⁴ 

[1] INRA, UMR 1083 Sciences pour l'OEnologie, 34060, Montpellier, France;INRA, UMR 1097 Diversité et Adaptation des Plantes Cultivées, 34060, Montpellier, France;INRA, UMR 1097 Diversité et Adaptation des Plantes Cultivées, 34060, Montpellier, France;Department of Ecology and Evolutionary Biology, Brown University, 02912, Providence, RI, USA;INRA, UMR 1131 Santé de la Vigne et Qualité du Vin, Université de Strasbourg, UMR 1131, F-68000, 67000, Colmar, Strasbourg, France;

关键词: Hairy Root;    Anthocyanin Content;    Anthocyanin Biosynthesis;    Anthocyanin Accumulation;    Grape Berry;   

DOI  :  10.1186/1471-2229-11-179

 received in 2011-06-21, accepted in 2011-12-15,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundPlant color variation is due not only to the global pigment concentration but also to the proportion of different types of pigment. Variation in the color spectrum may arise from secondary modifications, such as hydroxylation and methylation, affecting the chromatic properties of pigments. In grapes (Vitis vinifera L.), the level of methylation modifies the stability and reactivity of anthocyanin, which directly influence the color of the berry. Anthocyanin methylation, as a complex trait, is controlled by multiple molecular factors likely to involve multiple regulatory steps.ResultsIn a Syrah × Grenache progeny, two QTLs were detected for variation in level of anthocyanin methylation. The first one, explaining up to 27% of variance, colocalized with a cluster of Myb-type transcription factor genes. The second one, explaining up to 20% of variance, colocalized with a cluster of O-methyltransferase coding genes (AOMT). In a collection of 32 unrelated cultivars, MybA and AOMT expression profiles correlated with the level of methylated anthocyanin. In addition, the newly characterized AOMT2 gene presented two SNPs associated with methylation level. These mutations, probably leading to a structural change of the AOMT2 protein significantly affected the enzyme specific catalytic efficiency for the 3'-O-methylation of delphinidin 3-glucoside.ConclusionWe demonstrated that variation in methylated anthocyanin accumulation is susceptible to involve both transcriptional regulation and structural variation. We report here the identification of novel AOMT variants likely to cause methylated anthocyanin variation. The integration of QTL mapping and molecular approaches enabled a better understanding of how variation in gene expression and catalytic efficiency of the resulting enzyme may influence the grape anthocyanin profile.

【 授权许可】

Unknown   
© Fournier-Level et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.