Protective effect of the omega-3 polyunsaturated fatty acids: Eicosapentaenoic acid/Docosahexaenoic acid 1:1 ratio on cardiovascular disease risk markers in rats

Lipids in Health and Disease

Research

Josep Lluís Torres¹  Eunice Molinar-Toribio¹  Jara Pérez-Jiménez¹  Lucía Méndez²  José M Gallardo²  Isabel Medina²  Manuel Pazos²  M Rosa Nogués³  Laura Lluís³  Núria Taltavull³  Vanesa Sánchez-Martos³  Mònica Muñoz-Cortés³  Marta Romeu³  Montse Giralt³ 

[1]Institute of Advanced Chemistry of Catalonia –CSIC (IQAC-CSIC), Barcelona, Spain

[2]Institute of Marine Research-CSIC (IIM-CSIC), Vigo, Spain

[3]Unit of Pharmacology, Faculty of Medicine and Health Sciences, Rovira i Virgili University, Sant Llorenç, 21, 43201, Reus, Spain

关键词: Omega-3 polyunsaturated fatty acids (PUFA);    Eicosapentaenoic acid (EPA);    Docosahexaenoic acid (DHA);    Fish oils;    Oxidative stress;    Antioxidant status;    Cardiovascular disease risk;    Insulin resistance;   

DOI  :  10.1186/1476-511X-12-140

 received in 2013-07-23, accepted in 2013-09-24,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundHigh consumption of fish carries a lower risk of cardiovascular disease as a consequence of dietary omega-3 long chain polyunsaturated fatty acid (n-3 PUFA; especially EPA and DHA) content. A controversy exists about the component/s responsible of these beneficial effects and, in consequence, which is the best proportion between both fatty acids. We sought to determine, in healthy Wistar rats, the proportions of EPA and DHA that would induce beneficial effects on biomarkers of oxidative stress, and cardiovascular disease risk.MethodsFemale Wistar rats were fed for 13 weeks with 5 different dietary supplements of oils; 3 derived from fish (EPA/DHA ratios of 1:1, 2:1, 1:2) plus soybean and linseed as controls. The activities of major antioxidant enzymes (SOD, CAT, GPX, and GR) were determined in erythrocytes and liver, and the ORAC test was used to determine the antioxidant capacity in plasma. Also measured were: C reactive protein (CRP), endothelial dysfunction (sVCAM and sICAM), prothrombotic activity (PAI-1), lipid profile (triglycerides, cholesterol, HDLc, LDLc, Apo-A1, and Apo-B100), glycated haemoglobin and lipid peroxidation (LDL-ox and MDA values).ResultsAfter three months of nutritional intervention, we observed statistically significant differences in the ApoB100/ApoA1 ratio, glycated haemoglobin, VCAM-1, SOD and GPx in erythrocytes, ORAC values and LDL-ox. Supplementation with fish oil derived omega-3 PUFA increased VCAM-1, LDL-ox and plasma antioxidant capacity (ORAC). Conversely, the ApoB100/ApoA1 ratio and percentage glycated haemoglobin decreased.ConclusionsOur results showed that a diet of a 1:1 ratio of EPA/DHA improved many of the oxidative stress parameters (SOD and GPx in erythrocytes), plasma antioxidant capacity (ORAC) and cardiovascular risk factors (glycated haemoglobin) relative to the other diets.

【 授权许可】

Unknown   
© Lluís et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.