RNA-seq analysis of Pichia anomala reveals important mechanisms required for survival at lowpH

Microbial Cell Factories

Research

Amir Feizi¹  Eugene Fletcher¹  Verena Siewers¹  Jens Nielsen²  SungSoo Kim³ 

[1] Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 412 96, Gothenburg, Sweden;Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Kemivägen 10, 412 96, Gothenburg, Sweden;Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 412 96, Gothenburg, Sweden;Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Kemivägen 10, 412 96, Gothenburg, Sweden;Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2970, Hørsholm, Denmark;Samsung Advanced Institute of Technology, 130 Samsung-Ro YoungTong-Ku, Suwon, Kyunggi-do, South Korea;Biotech Research Team, Dongbu Farm Hannong Co., Ltd., 305-708, Daejeon, South Korea;

关键词: Pichia anomala;    Low pH;    Stress;    Biochemicals;    RNA-seq;    Genome sequencing;    ATP production;   

DOI  :  10.1186/s12934-015-0331-4

 received in 2015-06-12, accepted in 2015-08-31,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe product yield and titers of biological processes involving theconversion of biomass to desirable chemicals can be limited by environmentalstresses encountered by the microbial hosts used for the bioconversion. One ofthese main stresses is growth inhibition due to exposure to low pH conditions.In order to circumvent this problem, understanding the biological mechanismsinvolved in acid stress response and tolerance is essential. Characterisation ofwild yeasts that have a natural ability to resist such harsh conditions willpave the way to understand the biological basis underlying acid stressresistance. Pichia anomala possesses a uniqueability to adapt to and tolerate a number of environmental stresses particularlylow pH stress giving it the advantage to outcompete other microorganisms undersuch conditions. However, the genetic basis of this resistance has not beenpreviously studied.ResultsTo this end, we isolated an acid resistant strain of P. anomala, performed a gross phenotypiccharacterisation at low pH and also performed a whole genome and total RNAsequencing. By integrating the RNA-seq data with the genome sequencing data, wefound that several genes associated with different biological processesincluding proton efflux, the electron transfer chain and oxidativephosphorylation were highly expressed in P.anomala cells grown in low pH media. We therefore present datasupporting the notion that a high expression of proton pumps in the plasmamembrane coupled with an increase in mitochondrial ATP production enables thehigh level of acid stress tolerance of P.anomala.ConclusionsOur findings provide insight into the molecular and genetic basis oflow pH tolerance in P. anomala which waspreviously unknown. Ultimately, this is a step towards developingnon-conventional yeasts such as P. anomalafor the production of industrially relevant chemicals under low pHconditions.

【 授权许可】

CC BY   
© Fletcher et al. 2015