Genetic sex separation of the malaria vector, Anopheles arabiensis, by exposing eggs to dieldrin

Malaria Journal

Research

Mark Q Benedict¹  Jeremie RL Gilles²  Sharon M Soliban²  Hanano Yamada²  Clelia F Oliva³  Colin A Malcolm⁴ 

[1] Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, Via del Giochetto, 06122, Perugia, Italy;Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, Austria;Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, Austria;Maladies Infectieuses et Vecteurs: Ecologie, Genétique, Evolution et Contrôle (MIVEGEC), UMR5290 CNRS-IRD-Université de Montpellier I, Université de Montpellier II, 911 avenue Agropolis, 34394, Montpellier, France;Centre de Recherche et de Veille sur les Maladies Emergentes dans l’Océan Indien, Sainte Clotilde, La, Réunion;The School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, E1 4NS, London, UK;

关键词: Genetic sexing;    Anopheles arabiensis;    Sterile insect technique;    Dieldrin resistance;    Sterility;   

DOI  :  10.1186/1475-2875-11-208

 received in 2012-01-26, accepted in 2012-06-19,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe sterile insect technique (SIT) has been used with success for suppressing or eliminating important insect pests of agricultural or veterinary importance. In order to develop SIT for mosquitoes, female elimination prior to release is essential as they are the disease-transmitting sex. A genetic sexing strain (GSS) of Anopheles arabiensis was created based on resistance to dieldrin, and methods of sex separation at the egg stage were developed. The use of this strain for SIT will require sexually sterile males: useful radiation doses for this purpose were determined for pupae and adults.MethodsFor the creation of the sexing strain, dieldrin-resistant males were irradiated with 40 Gy using a 60Co source and were subsequently crossed to homozygous susceptible virgin females. Individual families were screened for semi-sterility and for male resistance to dieldrin. For sex separation, eggs of a resulting GSS, ANO IPCL1, were exposed to varying concentrations of dieldrin for different durations. Percent hatch, larval survival, and male and female emergence were recorded. Radiation induced sterility was determined following adult and pupa exposure to gamma rays at 0–105 Gy. Mortality induced by dieldrin treatment, and levels of sterility post radiation were investigated.ResultsANO IPCL1 contains a complex chromosome aberration that pseudo-links the male-determining Y chromosome and dieldrin resistance, conferring high natural semi-sterility. Exposure of eggs to 2, 3, and 4 ppm dieldrin solutions resulted in complete female elimination without a significant decrease of male emergence compared to the controls. A dose of 75 Gy reduced the fertility to 3.8 and 6.9% when males were irradiated as pupae or adults respectively, but the proportions of progeny of these males reaching adulthood were 0.6 and 1.5% respectivelyConclusionThe GSS ANO IPCL1 was shown to be a suitable strain for further testing for SIT though high semi-sterility is a disadvantage for mass rearing.

【 授权许可】

Unknown   
© Yamada et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.