Autotoxicity mechanism of Oryza sativa: transcriptome response in rice roots exposed to ferulic acid

BMC Genomics

Research Article

Shih-Feng Fu¹  Hao-Jen Huang²  Wen-Chang Chi²  Ngoc Nam Trinh²  Yu-Chywan Hsiung²  Ying-Chih Chen²  Yun-An Chen³  Chang-Hung Chou⁴ 

[1] Department of Biology, National Changhua University of Education, No. 1, Jin-De Road, 500, Changhua City, Taiwan, ROC;Department of Life Sciences, National Cheng Kung University, No. 1 University Rd. 701, Tainan, Taiwan, ROC;Department of Life Sciences, National Cheng Kung University, No. 1 University Rd. 701, Tainan, Taiwan, ROC;Department of Biological Sciences, National Sun Yat-Sen University, No. 70, Lienhai Rd., 80424, Kaohsiung, Taiwan, ROC;Graduate Institute of Ecology and Evolutionary Biology, College of Life Sciences, China Medical University, 91, Hsueh-Shih Road, 404, Taichung, Taiwan, ROC;

关键词: Allelochemical;    Ferulic acid;    Microarray;    Protein kinase;    Rice;    Autotoxicity;   

DOI  :  10.1186/1471-2164-14-351

 received in 2012-05-25, accepted in 2013-05-02,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAutotoxicity plays an important role in regulating crop yield and quality. To help characterize the autotoxicity mechanism of rice, we performed a large-scale, transcriptomic analysis of the rice root response to ferulic acid, an autotoxin from rice straw.ResultsRoot growth rate was decreased and reactive oxygen species, calcium content and lipoxygenase activity were increased with increasing ferulic acid concentration in roots. Transcriptome analysis revealed more transcripts responsive to short ferulic-acid exposure (1- and 3-h treatments, 1,204 genes) than long exposure (24 h, 176 genes). Induced genes were involved in cell wall formation, chemical detoxification, secondary metabolism, signal transduction, and abiotic stress response. Genes associated with signaling and biosynthesis for ethylene and jasmonic acid were upregulated with ferulic acid. Ferulic acid upregulated ATP-binding cassette and amino acid/auxin permease transporters as well as genes encoding signaling components such as leucine-rich repeat VIII and receptor-like cytoplasmic kinases VII protein kinases, APETALA2/ethylene response factor, WRKY, MYB and Zinc-finger protein expressed in inflorescence meristem transcription factors.ConclusionsThe results of a transcriptome analysis suggest the molecular mechanisms of plants in response to FA, including toxicity, detoxicification and signaling machinery. FA may have a significant effect on inhibiting rice root elongation through modulating ET and JA hormone homeostasis. FA-induced gene expression of AAAP transporters may contribute to detoxicification of the autotoxin. Moreover, the WRKY and Myb TFs and LRR-VIII and SD-2b kinases might regulate downstream genes under FA stress but not general allelochemical stress. This comprehensive description of gene expression information could greatly facilitate our understanding of the mechanisms of autotoxicity in plants.

【 授权许可】

Unknown   
© Chi et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.