Prevalence and characterization of extended-spectrum beta-lactamases producing Enterobacteriaceae in healthy children and associated risk factors

Annals of Clinical Microbiology and Antimicrobials

Research

F. M. Ali¹  M. A. Fawzi²  S. M. Hijazi³  K. H. Abd El Galil⁴ 

[1] Department of Microbiology and Immunology, Infection Control, Faculty of Medicine, Ain Shams University, Cairo, Egypt;Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt;Department of Pharmaceutical Sciences (Pharmaceutical Microbiology), Faculty of Pharmacy, Beirut Arab University, Beirut, Lebanon;Department of Pharmaceutical Sciences (Pharmaceutical Microbiology), Faculty of Pharmacy, Beirut Arab University, Beirut, Lebanon;Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt;

关键词: Extended-spectrum beta-lactamase;    Children;    TEM;    SHV;    CTX-M;    CTX-9;    Dairy products;    Meat;    Lebanon;   

DOI  :  10.1186/s12941-016-0121-9

 received in 2015-06-30, accepted in 2016-01-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCommunity acquired infections due to extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) had been increased. The fecal flora of children in the community represents a huge potential reservoir for ESBLs which are located on highly transmissible plasmids. This study examined the prevalence of ESBL-PE fecal carriage, antimicrobial susceptibility pattern, possible risk factors, and characterized the genes encoding these ESBL enzymes in Lebanese children community.MethodsA total of 125 rectal swabs were taken from healthy children aged from 1 to 5 years. Detection of ESBLs was carried out using combination-disc method test and multiplex PCR. A questionnaire concerning child’s lifestyle and risk factors for ESBL carriage was illustrated.ResultsThirty-one of 125 participants (24.8 %) carried ESBL-PE. Regular consumption of meat, and chicken were significantly associated with high carriage rate of ESBL-PE, while dairy products (milk, yogurt, cheese) association was non-significant. Intimate hygiene habits were found also affecting the carriage rate. Multiple bla genes were the most common, 48.4 % (15/31) of ESBL-PE carried both blaCTX-M and blaTEM, and 22.6 % (7/31) carried blaCTX-M, blaSHV, and blaTEM, 29 % (9) carried blaCTX-M only. Concerning CTX-M-types, CTX-M-9 was the most predominant (24/31) and mostly in combination with CTX-M-15 type.ConclusionHigh rate of colonization in healthy children with ESBL-PE was observed, regular consumption of dietary products from animal source (meat or chicken) were associated with this colonization in the community in non-hospitalized children. To our best knowledge it is the first study about regular consumption of dairy product as a risk factor for ESBL-PE community carriage, the first data about the carriage rate of ESBL-PE in community children in Lebanon and Middle East, and for the wide dissemination of CTX-M-9 type in this population.

【 授权许可】

CC BY   
© Hijazi et al. 2016