Genotypic detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae in a Tertiary care hospital

International Journal of Biomedical and Advance Research

Fouzia Begum¹  A. S. Damle¹ 

关键词: ESBL;    Klebsiellapneumoniae;    TEM;    SHV;   

DOI  :  10.7439/ijbar.v6i2.1503

学科分类：药学、药理学、毒理学（综合）

来源: Scholar Science Journals

【 摘 要 】

Aim : This? study? was? carried? out? to? determine? the? presence? of? TEM? and? SHV? genes? in? extended-spectrum? ?-lactamase? (ESBL)? producing?Klebsiella ?pneumoniae .? The? study? was? also? aimed? to? compare? results? of? PCDDT? and? genotypic? methods. Information on molecular types of ESBL positiveKlebsiellaspecies is less from India; lesser still from this area of Marathwada, a part of Maharashtra state. Materials? and? Methods:?A? total? of? 340? strains? of?Klebsiella? pneumoniae?were? selected? for? the? study? from? June? 2013-December? 2013.? Kirby ?Bauer? disk? diffusion? method? was? performed? to? determine? the? antibiotic? resistance? pattern.? Screened? for? ESBL? and? confirmed? by? phenotypic? confirmatory? disc? diffusion? test? (PCDDT).? 100? randomly? selected? isolates? were investigated? for? the? presence? of? TEM? and? SHV? genes? via? Polymerase? chain? reaction? (PCR)? using? two? different? sets? of? primers.? Multiplex? PCR? was? also? performed? for? the? same.? Results:Phenotypic? confirmatory? test? was? able? to? detect? ESBL? production? in? 90%? of?Klebsiella? pneumoniae?isolates.? Among? the? two? ESBL? genotypes,? the? most? prevalent? genotype? was? found? to? be? TEM.? Majority? of? ESBL? producing? isolates? possess? both? ESBL? genes.? Conclusion:?Multiplex? PCR? can? be? used? as? a? rapid? method? to? identify? common? genes? (TEM? and? SHV)? responsible? for? extended? spectrum? beta? lactamase? production? in?Klebsiella? pneumoniae.?It? will? prove? valuable? for? surveillance? and? for? determining? the? line? of? treatment? against? drug? resistant? organisms,? thus? saving? precious? time? and? resources.? PCDDT? results? correlated? with? genotypic? method? in? all? the? tested? strains.

【 授权许可】

CC BY