Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles

Journal of Nanobiotechnology

Research

Sang Rye Park¹  Ji Young Kim¹  Young Chan Jeon²  Hyun Wook Lee³  Hae June Lee⁴  Jin Woo Hong⁵  Byul bo-ra Choi⁶  Gyoo Cheon Kim⁶ 

[1] Department of Dental Hygiene, Kyungnam College of Information and Technology, 617-701, Busan, Rep. Korea;Department of Dental Prosthetics, School of Dentistry, Pusan National University, 602-739, Yangsan, Republic of Korea;Department of Electrical Engineering, Pohang University of Science and Technology, 790-784, Pohang, Rep. Korea;Department of Electronics Engineering, Pusan National University, 609-735, Busan, Rep. Korea;Department of Korean Internal Medicine, School of Korean Medicine, Pusan National University, 626-870, Yangsan, Korea;Department of Oral Anatomy, School of Dentistry, Pusan National University, 602-739, Yangsan, Rep. Korea;

关键词: Gold nanoparticle;    Low-temperature plasma;    Streptococcus mutans;    Sterilization;    Oral care;   

DOI  :  10.1186/s12951-014-0029-5

 received in 2014-05-22, accepted in 2014-08-01,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundRecently, non-thermal atmospheric pressure plasma sources have been used for biomedical applications such as sterilization, cancer treatment, blood coagulation, and wound healing. Gold nanoparticles (gNPs) have unique optical properties and are useful for biomedical applications. Although low-temperature plasma has been shown to be effective in killing oral bacteria on agar plates, its bactericidal effect is negligible on the tooth surface. Therefore, we used 30-nm gNPs to enhance the killing effect of low-temperature plasma on human teeth.ResultsWe tested the sterilizing effect of low-temperature plasma on Streptococcus mutans (S. mutans) strains. The survival rate was assessed by bacterial viability stains and colony-forming unit counts. Low-temperature plasma treatment alone was effective in killing S. mutans on slide glasses, as shown by the 5-log decrease in viability. However, plasma treatment of bacteria spotted onto tooth surface exhibited a 3-log reduction in viability. After gNPs were added to S. mutans, plasma treatment caused a 5-log reduction in viability, while gNPs alone did not show any bactericidal effect. The morphological changes in S. mutans caused by plasma treatment were examined by transmission electron microscopy, which showed that plasma treatment only perforated the cell walls, while the combination treatment with plasma and gold nanoparticles caused significant cell rupture, causing loss of intracellular components from many cells.ConclusionsThis study demonstrates that low-temperature plasma treatment is effective in killing S. mutans and that its killing effect is further enhanced when used in combination with gNPs.

【 授权许可】

Unknown   
© Park et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.