Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles

Journal of Nanobiotechnology

Young Chan Jeon⁴  Gyoo Cheon Kim⁵  Byul bo-ra Choi⁵  Ji Young Kim¹  Hae June Lee⁶  Jin Woo Hong²  Hyun Wook Lee³  Sang Rye Park¹ 

[1] Department of Dental Hygiene, Kyungnam College of Information and Technology, Busan 617-701, Rep. Korea;Department of Korean Internal Medicine, School of Korean Medicine, Pusan National University, Yangsan 626-870, Korea;Department of Electrical Engineering, Pohang University of Science and Technology, Pohang 790-784, Rep. Korea;Department of Dental Prosthetics, School of Dentistry, Pusan National University, Yangsan 602-739, Republic of Korea;Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan 602-739, Rep. Korea;Department of Electronics Engineering, Pusan National University, Busan 609-735, Rep. Korea

关键词: Oral care;    Sterilization;    Streptococcus mutans;    Low-temperature plasma;    Gold nanoparticle;   

Others  :  1146385
DOI  :  10.1186/s12951-014-0029-5

 received in 2014-05-22, accepted in 2014-08-01,  发布年份 2014

【 摘 要 】

Background

Recently, non-thermal atmospheric pressure plasma sources have been used for biomedical applications such as sterilization, cancer treatment, blood coagulation, and wound healing. Gold nanoparticles (gNPs) have unique optical properties and are useful for biomedical applications. Although low-temperature plasma has been shown to be effective in killing oral bacteria on agar plates, its bactericidal effect is negligible on the tooth surface. Therefore, we used 30-nm gNPs to enhance the killing effect of low-temperature plasma on human teeth.

Results

We tested the sterilizing effect of low-temperature plasma on Streptococcus mutans (S. mutans) strains. The survival rate was assessed by bacterial viability stains and colony-forming unit counts. Low-temperature plasma treatment alone was effective in killing S. mutans on slide glasses, as shown by the 5-log decrease in viability. However, plasma treatment of bacteria spotted onto tooth surface exhibited a 3-log reduction in viability. After gNPs were added to S. mutans, plasma treatment caused a 5-log reduction in viability, while gNPs alone did not show any bactericidal effect. The morphological changes in S. mutans caused by plasma treatment were examined by transmission electron microscopy, which showed that plasma treatment only perforated the cell walls, while the combination treatment with plasma and gold nanoparticles caused significant cell rupture, causing loss of intracellular components from many cells.

Conclusions

This study demonstrates that low-temperature plasma treatment is effective in killing S. mutans and that its killing effect is further enhanced when used in combination with gNPs.

【 授权许可】

   
2014 Park et al.; licensee BioMed Central Ltd.

【 图 表 】

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