Mitochondrial genetic differentiation across populations of the malaria vector Anopheles lesteri from China (Diptera: Culicidae)

Malaria Journal

Research

Jing Wu¹  Manni Yang¹  Yajun Ma¹ 

[1] Department of Pathogen Biology, Second Military Medical University, 200433, Shanghai, China;

关键词: Malaria;    Population Genetic Structure;    Vector Population;    Mismatch Distribution;    COII Gene;   

DOI  :  10.1186/1475-2875-10-216

 received in 2011-01-27, accepted in 2011-08-03,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundAnopheles lesteri is a primary vector of Plasmodium spp. in central China. A complete understanding of vector population structure and the processes responsible for the differentiation is important to the vector-based malaria control programmes and for identifying heterogeneity in disease transmission as a result of discrete vector populations. There is no adequate An. lesteri population genetic data available.MethodsPolymorphism of sequence variations in mitochondrial COII and Cytb genes were assessed to explore the level of genetic variability and differentiation among six populations of An. lesteri from China.ResultsThere were 30 (4.37%) and 21 (5.33%) polymorphic sites for mtDNA-COII and Cytb gene, respectively. Totally 31 COII and 30 Cytb haplotypes were obtained. The range of FST values was from 0.101 to 0.655 by mtDNA-COII, and 0.029 to 0.231 by Cytb gene. The analysis of molecular variance (AMOVA) showed that the percentage of variation within populations (65.83%, 88.48%) was greater than that among populations (34.17%, 11.52%) using both genes. The Tajima's D and Fu's Fs values were all negative, except Tajima's D values of YN and HNB populations, which suggest a large number of low-frequency mutations in populations and the populations were in expansion proceeding.ConclusionsLevels of genetic variation within An. lesteri populations were higher than among them. While these results may suggest considerable levels of gene flow, other explanations, such as the effect of historical population perturbations can also be hypothesized.

【 授权许可】

Unknown   
© Yang et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.