Illness progression in chronic fatigue syndrome: a shifting immune baseline

BMC Immunology

Research Article

Henrique Fernandes¹  AnneLiese Smylie¹  Lindsey Russell¹  Gordon Broderick²  Elizabeth G. Balbin³  Jeanna Harvey⁴  Zachary Barnes⁵  Renee Taylor⁶  Ben Z. Katz⁷  Fanny Collado⁸  Mary Ann Fletcher⁹  Nancy G. Klimas⁹ 

[1] Department of Medicine, University of Alberta, Edmonton, AB, Canada;Department of Medicine, University of Alberta, Edmonton, AB, Canada;Miami Veterans Affairs Medical Center, Miami, FL, USA;Institute for Neuro-immune Medicine, Nova Southeastern University, Suite 3440 University Park Plaza, 3424 South University Drive, 33328, Fort Lauderdale, FL, USA;Department of Medicine, University of Miami, Miami, FL, USA;Department of Medicine, University of Miami, Miami, FL, USA;Miami Veterans Affairs Medical Center, Miami, FL, USA;Department of Medicine, University of Miami, Miami, FL, USA;Miami Veterans Affairs Medical Center, Miami, FL, USA;Institute for Neuro-immune Medicine, Nova Southeastern University, Suite 3440 University Park Plaza, 3424 South University Drive, 33328, Fort Lauderdale, FL, USA;Department of Occupational Therapy, University of Illinois at Chicago, Chicago, IL, USA;Division of Infectious Diseases, Ann & Robert H Lurie Children’s Hospital of Chicago, Chicago, IL, USA;Miami Veterans Affairs Medical Center, Miami, FL, USA;Miami Veterans Affairs Medical Center, Miami, FL, USA;Institute for Neuro-immune Medicine, Nova Southeastern University, Suite 3440 University Park Plaza, 3424 South University Drive, 33328, Fort Lauderdale, FL, USA;

关键词: Cytokines;    Chronic fatigue;    Menopause and immunity;    Immune signaling;    Classification model;   

DOI  :  10.1186/s12865-016-0142-3

 received in 2015-08-07, accepted in 2016-02-29,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundValidation of biomarkers for myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) across data sets has proven disappointing. As immune signature may be affected by many factors, our objective was to explore the shift in discriminatory cytokines across ME/CFS subjects separated by duration of illness.MethodsCytokine expression collected at rest across multiple studies for female ME/CFS subjects (i) 18 years or younger, ill for 2 years or less (n = 18), (ii) 18–50 years of age, ill for 7 years (n = 22), and (iii) age 50 years or older (n = 28), ill for 11 years on average. Control subjects were matched for age and body mass index (BMI). Data describing the levels of 16 cytokines using a chemiluminescent assay was used to support the identification of separate linear classification models for each subgroup. In order to isolate the effects of duration of illness alone, cytokines that changed significantly with age in the healthy control subjects were excluded a priori.ResultsOptimal selection of cytokines in each group resulted in subsets of IL-1α, 6, 8, 15 and TNFα. Common to any 2 of 3 groups were IL-1α, 6 and 8. Setting these 3 markers as a triple screen and adjusting their contribution according to illness duration sub-groups produced ME/CFS classification accuracies of 75–88 %. The contribution of IL-1α, higher in recently ill adolescent ME/CFS subjects was progressively less important with duration. While high levels of IL-8 screened positive for ME/CFS in the recently afflicted, the opposite was true for subjects ill for more than 2 years. Similarly, while low levels of IL-6 suggested early ME/CFS, the reverse was true in subjects over 18 years of age ill for more than 2 years.ConclusionsThese preliminary results suggest that IL-1α, 6 and 8 adjusted for illness duration may serve as robust biomarkers, independent of age, in screening for ME/CFS.

【 授权许可】

CC BY   
© Russell et al. 2016