Evidence and consequences of self-fertilisation in the predominantly outbreeding forage legume Onobrychis viciifolia

BMC Genetics

Research Article

Achim Walter¹  Christoph Grieder²  Franco Widmer³  Sonja Reinhard³  Roland Kölliker³  Katharina Kempf⁴ 

[1] Crop Science, ETH Zurich, Universitätstrasse 2, 8092, Zurich, Switzerland;Fodder Plant Breeding, Agroscope Reckenholz ISS, Reckenholzstrasse 191, 8046, Zurich, Switzerland;Molecular Ecology, Agroscope Reckenholz ISS, Reckenholzstrasse 191, 8046, Zurich, Switzerland;Molecular Ecology, Agroscope Reckenholz ISS, Reckenholzstrasse 191, 8046, Zurich, Switzerland;Crop Science, ETH Zurich, Universitätstrasse 2, 8092, Zurich, Switzerland;

关键词: Onobrychis viciifolia;    Sainfoin;    Self-fertilisation;    Inbreeding depression;    SRAP marker;    SSR marker;    Tetraploidy;    Outbreeding;   

DOI  :  10.1186/s12863-015-0275-z

 received in 2015-07-06, accepted in 2015-10-02,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundSainfoin (Onobrychis viciifolia) is a promising alternative forage plant of good quality, moderate nutrient demand and a high content of polyphenolic compounds. Its poor adoption is caused by the limited availability of well performing varieties. Sainfoin is characterised as tetraploid and mainly outcrossing, but the extent of self-fertilisation and its consequences was not investigated so far. This study aimed at assessing the rate of self-fertilisation in sainfoin under different pollination regimes and at analysing the consequences on plant performance in order to assist future breeding efforts.MethodsThe self-fertilisation rate was assessed in three sainfoin populations with artificially directed pollination (ADP) and in three populations with non-directed pollination (NDP). Dominant SRAP (sequence-related amplified polymorphism) and codominant SSR (simple sequence repeats) markers were used to detect self-fertilisation in sainfoin for the first time based on molecular marker data.ResultsHigh rates of self-fertilisation of up to 64.8 % were observed for ADP populations in contrast to only up to 3.9 % for NDP populations. Self-fertilisation in ADP populations led to a reduction in plant height, plant vigour and, most severely, for seed yield.ConclusionsAlthough sainfoin is predominantly outcrossing, self-fertilisation can occur to a high degree under conditions of limited pollen availability. These results will influence future breeding efforts because precautions have to be taken when crossing breeding material. The resulting inbreeding depression can lead to reduced performance in self-fertilised offspring. Nevertheless the possibility of self-fertilisation also offers new ways for hybrid breeding based on the development of homogenous inbred lines.

【 授权许可】

CC BY   
© Kempf et al. 2015