MEK5 overexpression is associated with the occurrence and development of colorectal cancer

BMC Cancer

Research Article

Zhiqiang Chen¹  Wei Wang¹  Dechang Diao¹  Liaonan Zou¹  Jin Wan¹  Junsheng Peng²  Xinlin Chen³  Lei Wang⁴  Huanliang Liu⁵ 

[1] Department of Gastrointestinal Surgery, Guangdong Provincal Hospital of Traditional Chinese Medicine, 510120, Guangdong, China;Department of Gastrointestinal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, 510655, Guangzhou, Guangdong, China;Department of Preventive Medicine and Medical Statistics, College of Fundamental Medical Science, Guangzhou University of Traditional Chinese Medicine, 510006, Guangdong, China;Institute of Gastroenterology, Sun Yat-Sen University, 510655, Guangzhou, China;Department of Gastrointestinal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, 510655, Guangzhou, Guangdong, China;Institute of Gastroenterology, Sun Yat-Sen University, 510655, Guangzhou, China;Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, 510080, Guangdong, China;

关键词: MEK5;    Colorectal cancer;    Univariate analyses;    RNA interference;    Tumor growth;   

DOI  :  10.1186/s12885-016-2327-9

 received in 2015-12-08, accepted in 2016-04-20,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMitogen/extracellular signal-regulated kinase kinase-5 (MEK5) has been confirmed to play a pivotal role in tumor carcinogenesis and progression. However, few studies have investigated the role of MEK5 in colorectal cancer (CRC).MethodsMEK5 expression was determined by immunohistochemistry (IHC) in tissue microarrays (TMAs) containing 2 groups of tissues, and western blotting was used to confirm MEK5 expression in 8 cases of primary CRC tissues and paired normal mucosa. RNA interference was used to verify the biological function of MEK5 gene in the development of CRC.ResultsIHC revealed the expression of MEK5 was higher in tumor tissues (38.1 %), compared with adjacent normal tissue (8.3 %). Western blot showed that, MEK5 expression was upregulated in CRC tumor tissues compared with normal tissue. Analysis of clinical pathology parameters indicated MEK5 overexpression was significantly correlated with the depth of invasion, lymph node metastasis, distant metastasis and histological grade. Survival analysis revealed that MEK5 overexpression negatively correlated with cancer-free survival (hazard ratio 1.64, P = 0.017). RNA interference-mediated knockdown of MEK5 in SW480 colon cancer cells decreased their proliferation, division, migration and invasiveness in vitro and slowed down tumors growth in mice engrafted with the cells.ConclusionMEK5 plays an important role in CRC progression and may be a potential molecular target for the treatment of CRC.

【 授权许可】

CC BY   
© Diao et al. 2016