Circulating LH/hCG receptor (LHCGR) may identify pre-treatment IVF patients at risk of OHSS and poor implantation

Reproductive Biology and Endocrinology

Research

Anne E Chambers¹  Subhasis Banerjee¹  Krishnaveni P Nayini²  Gillian M Lockwood²  Walter E Mills³ 

[1] Department of Clinical Biochemistry, Heartlands Hospital, B9 5SS, Birmingham, UK;Origin Biomarkers, BioPark, Broadwater Road, AL7 3AX, Welwyn Garden City, Hertfordshire, UK;Midland Fertility Services, WS9 8LT, Aldridge, UK;Origin Biomarkers, BioPark, Broadwater Road, AL7 3AX, Welwyn Garden City, Hertfordshire, UK;

关键词: soluble LHCGR;    LH-LHCGR;    AMH;    FSH;    OHSS;    IVF;    Pregnancy;   

DOI  :  10.1186/1477-7827-9-161

 received in 2011-11-01, accepted in 2011-12-23,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSuccessful pregnancy via in vitro fertilization (IVF) depends on the recovery of an adequate number of healthy oocytes and on blastocyst implantation following uterine transfer. Two hormones, LH and hCG, utilize a common LH/hCG receptor (LHCGR), variations in which have profound implications in human reproduction. Soluble LHCGR (sLHCGR) is released from experimental cell lines and placental explants and it can be detected in the follicular fluid and serum.MethodsTo evaluate the impact of circulating soluble LHCGR (sLHCGR) in fertility treatment, we measured sLHCGR and LH-sLHCGR complex in serum from women seeking IVF using specifically developed quantitative enzyme-linked immunosorbent assays (ELISA). Following an IVF cycle of treatment, patients were grouped according to oocyte yield into low (lower than or equal to 7 oocytes), intermediate (8-14 oocytes) and high (greater than or equal to 15 oocytes) responders and pregnancy outcome noted.ResultsPre-treatment sLHCGR identified many women at risk of ovarian hyperstimulation. Low levels of sLHCGR were associated with pregnancy in both high and low responders but sLHCGR did not significantly affect the treatment outcome of intermediate responders. Low responders who failed to become pregnant had high levels of circulating sLHCGR bound to LH (LH-sLHCGR).ConclusionsPre-treatment measurement of sLHCGR could be used to tailor individual fertility treatment programs and improve outcomes by avoiding ovarian hyperstimulation and poor embryo implantation.

【 授权许可】

Unknown   
© Chambers et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.