Imbalance in superoxide dismutase/thioredoxin reductase activities in hypercholesterolemic subjects: relationship with low density lipoprotein oxidation

Lipids in Health and Disease

Research

Marta Medeiros Frescura Duarte¹  Paula Rossini Augusti²  Sabrina Somacal³  Andréia Quatrin³  Tatiana Emanuelli³  Amanda Roggia Ruviaro³  Juliana Tanara Vicentini³  Greicy Michelle Marafiga Conterato³ 

[1] Departament of Health Sciences, Lutheran University of Brazil, Santa Maria, RS, Brazil;Department of Biochemistry, Graduate Program on Biological Sciences/Biochemistry, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil;Campus Itaqui, Federal University of Pampa, Itaqui, RS, Brazil;Department of Food Technology and Science, Integrated Center for Laboratory Analysis Development (NIDAL), Center of Rural Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil;

关键词: Atherogenic index;    Hypercholesterolemia;    Oxidized low density lipoprotein;    Superoxide dismutase;    Thioredoxin reductase;   

DOI  :  10.1186/1476-511X-11-79

 received in 2012-04-24, accepted in 2012-06-09,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThere is a relationship among hypercholesterolemia, oxidative stress and inflammation in the atherogenesis. Thus, the objective of the present study was to assess paraoxonase (PON1), superoxide dismutase (SOD) and thioredoxin reductase (TrxR-1) activities and their relationship with lipids, oxidative stress and inflammation in subjects with different low density lipoprotein-cholesterol (LDL) levels.MethodsSerum lipids, highly sensitive C-reactive protein (hs-CRP), lipid and protein oxidation, oxidized LDL (LDLox) and LDLox autoantibodies (LDLoxAB) levels and enzymes activities were measured in a total of 116 subjects that were divided into the following groups according to their LDL levels: low-LDL group (LDL < 100 mg/dL, n = 23), intermediate-LDL group (LDL 100–160 mg/dL, n = 50) and high-LDL group (LDL > 160 mg/dL, n = 43).ResultsThe LDLox and hs-CRP levels increased in the high-LDL group (2.7- and 3.7- fold, respectively), whereas the intermediate and high-LDL groups had higher LDLoxAB (2.2- and 3.1-fold) when compared to low-LDL group (p < 0.05). Similarly, SOD activity, the atherogenic index (AI) and protein oxidation were also higher in the intermediate (1.3-, 1.3- and 1.2-fold) and high-LDL (1.6-, 2.3- and 1.6-fold) groups when compared to the low-LDL group (p < 0.05). Lipid oxidation and SOD/TrxR-1 ratio increased only in the high-LDL group (1.3- and 1.6-fold) when compared to the low-LDL group (p < 0.05). The SOD/TrxR-1 ratio was positively correlated to TBARS (r = 0.23, p < 0.05), LDLox (r = 0.18, p < 0.05), LDLoxAB (r = 0.21, p < 0.05), LDL (r = 0.19, p < 0.05) and AI (r = 0.22, p < 0.05). PON1 and TrxR-1 activities were similar among groups.ConclusionsSome oxidative events initiate when LDL levels are clinically acceptable. Moreover, hypercholesterolemic patients have an imbalance in SOD and TrxR-1 activities that is positively associated to LDL oxidation.

【 授权许可】

Unknown   
© Augusti et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.