MicroRNA-217 functions as a prognosis predictor and inhibits colorectal cancer cell proliferation and invasion via an AEG-1 dependent mechanism

BMC Cancer

Research Article

Ke-wei Jiang¹  Yi-chao Yan¹  Zhi-dong Gao¹  Ji-zhun Zhang¹  Bo Wang¹  Zhan-long Shen¹  Chao Shen¹  Ying-jiang Ye¹  Yang Yang¹  Shan Wang¹  Chun-you Wang²  Gang Zhao² 

[1] Department of Gastroenterological Surgery, Peking University People’s Hospital, No.11 Xizhimen South Street, Xicheng District, 100044, Beijing, P.R. China;Pancreatic Disease Institute, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China;

关键词: miR-217;    AEG-1;    colorectal cancer;    proliferation;    invasion;   

DOI  :  10.1186/s12885-015-1438-z

 received in 2014-10-21, accepted in 2015-05-14,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundRecent studies have indicated the possible function of miR-217 in tumorigenesis. However, the roles of miR-217 in colorectal cancer (CRC) are still largely unknown.MethodsWe examined the expression of miR-217 and AEG-1 in 50 CRC tissues and the corresponding noncancerous tissues by qRT-PCR. The clinical significance of miR-217 was analyzed. CRC cell lines with miR-217 upregulation and AEG-1 silencing were established and the effects on tumor growth in vitro and in vivo were assessed. Dual-luciferase reporter gene assays were also performed to investigate the interaction between miR-217 and AEG-1.ResultsOur data demonstrated that miR-217 was significantly downregulated in 50 pairs of colorectal cancer tissues. MiR-217 expression levels were closely correlated with tumor differentiation. Moreover, decreased miR-217 expression was also associated with shorter overall survival of CRC patients. MiR-217 overexpression significantly inhibited proliferation, colony formation and invasiveness of CRC cells by promoting apoptosis and G0/G1 phase arrest. Interestingly, ectopic miR-217 expression decreased AEG-1 expression and repressed luciferase reporter activity associated with the AEG-1 3′-untranslated region (UTR). AEG-1 silencing resulted in similar biological behavior changes to those associated with miR-217 overexpression. Finally, in a nude mouse xenografted tumor model, miR-217 overexpression significantly suppressed CRC cell growth.ConclusionsOur findings suggest that miR-217 has considerable value as a prognostic marker and potential therapeutic target in CRC.

【 授权许可】

CC BY   
© Wang et al.; licensee BioMed Central. 2015