The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia

Lipids in Health and Disease

Research

Ahmed Bekir¹  Zied Zarai²  Hafedh Mejdoub³  Adel Kadri⁴  Néji Gharsallah⁵  Ines Ben Chobba⁵  Riadh Ben Mansour⁶ 

[1] Département de Génie des procédés, ISET Sfax, University of Sfax, Km 2,5 Rte de Mahdia, 3099, Sfax, Tunisia;Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, BPW, University of Sfax, 1173, Sfax, Tunisia;Laboratoire de Biochimie, Faculté des sciences de Sfax, University of Sfax, B.P. 1171, 3000Sfax, Tunisia;Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences de Sfax, University of Sfax, B.P. 1171, 3000, Sfax, Tunisia;Laboratoire de Microbiologie Alimentaire, Faculté des sciences de Sfax, University of Sfax, B.P. 1171, 3000, Sfax, Tunisia;Unité de recherche Biotechnologie et pathologies, Institut Supérieur de Biotechnologie de Sfax, University of Sfax, Tunisia;

关键词: Antimicrobial;    cytotoxicity;    essential oil;    Marrubium vulgare;    pathogenic microorganisms;    HeLa cell lines;   

DOI  :  10.1186/1476-511X-10-161

 received in 2011-08-23, accepted in 2011-09-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundIn order to validate its antiseptic and anticancer properties with respect to traditional uses, we have screened for the first time the antimicrobial activity of aerial parts of M. vulgare L. essential oil against different pathogenic microorganisms and the cytotoxic activity against HeLa cell lines.MethodsThe agar disk diffusion method was used to study the antibacterial activity of M. vulgare essential oil against 12 bacterial and 4 fungi strains. The disc diameters of zone of inhibition (DD), the minimum inhibitory concentrations (MIC) and the concentration inhibiting 50% (IC50) were investigated to characterize the antimicrobial activities of this essential oil. The in vitro cytotoxicity of M. vulgare essential oil was examined using a modified MTT assay; the viability and the IC50 were used to evaluate this test.ResultsThe antimicrobial activity of the essential oil was investigated in order to evaluate its efficacy against the different tested microorganisms. The present results results showed a significant activity against microorganisms especially Gram (+) bacteria with inhibition zones and minimal inhibitory concentration values in the range of 6.6-25.2 mm and 1120-2600 μg/ml, respectively, whereas Gram (-) bacteria exhibited a higher resistance. As far as the antifungal activity, among four strains tested, Botrytis cinerea exhibited the strongest activity with inhibition zones of 12.6 mm. However, Fusarium solani, Penicillium digitatum and Aspergillus niger were less sensitive to M. vulgare essential oil. About the citotoxicity assay, this finding indicate the capability of this essential oil to inhibited the proliferation of HeLa cell lines under some conditions with IC50 value of 0.258 μg/ml.ConclusionThis investigation showed that the M. vulgare essential oil has a potent antimicrobial activity against some Gram (+) pathogenic bacteria and Botrytis cinerea fungi. The present studies confirm the use of this essential oil as anticancer agent. Further research is required to evaluate the practical values of therapeutic applications.

【 授权许可】

Unknown   
© Zarai et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.