The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia

Lipids in Health and Disease

Néji Gharsallah⁵  Hafedh Mejdoub²  Ahmed Bekir⁶  Riadh Ben Mansour³  Ines Ben Chobba⁵  Adel Kadri¹  Zied Zarai⁴ 

[1] Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences de Sfax, B.P. 1171, 3000 Sfax, University of Sfax, Tunisia;Laboratoire de Biochimie, Faculté des sciences de Sfax, B.P. 1171, 3000 Sfax, University of Sfax, Tunisia;Unité de recherche Biotechnologie et pathologies, Institut Supérieur de Biotechnologie de Sfax, University of Sfax, Tunisia;Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, BPW, 1173 Sfax, University of Sfax, Tunisia;Laboratoire de Microbiologie Alimentaire, Faculté des sciences de Sfax, B.P. 1171, 3000 Sfax, University of Sfax Tunisia;Département de Génie des procédés, ISET Sfax, Km 2,5 Rte de Mahdia, 3099 Sfax, University of Sfax, Tunisia

关键词: HeLa cell lines;    pathogenic microorganisms;    Marrubium vulgare L.,;    essential oil;    cytotoxicity;    Antimicrobial;   

Others  :  1212513
DOI  :  10.1186/1476-511X-10-161

 received in 2011-08-23, accepted in 2011-09-21,  发布年份 2011

【 摘 要 】

Background

In order to validate its antiseptic and anticancer properties with respect to traditional uses, we have screened for the first time the antimicrobial activity of aerial parts of M. vulgare L. essential oil against different pathogenic microorganisms and the cytotoxic activity against HeLa cell lines.

Methods

The agar disk diffusion method was used to study the antibacterial activity of M. vulgare essential oil against 12 bacterial and 4 fungi strains. The disc diameters of zone of inhibition (DD), the minimum inhibitory concentrations (MIC) and the concentration inhibiting 50% (IC₅₀) were investigated to characterize the antimicrobial activities of this essential oil. The in vitro cytotoxicity of M. vulgare essential oil was examined using a modified MTT assay; the viability and the IC₅₀ were used to evaluate this test.

Results

The antimicrobial activity of the essential oil was investigated in order to evaluate its efficacy against the different tested microorganisms. The present results results showed a significant activity against microorganisms especially Gram (+) bacteria with inhibition zones and minimal inhibitory concentration values in the range of 6.6-25.2 mm and 1120-2600 μg/ml, respectively, whereas Gram (-) bacteria exhibited a higher resistance. As far as the antifungal activity, among four strains tested, Botrytis cinerea exhibited the strongest activity with inhibition zones of 12.6 mm. However, Fusarium solani, Penicillium digitatum and Aspergillus niger were less sensitive to M. vulgare essential oil. About the citotoxicity assay, this finding indicate the capability of this essential oil to inhibited the proliferation of HeLa cell lines under some conditions with IC₅₀ value of 0.258 μg/ml.

Conclusion

This investigation showed that the M. vulgare essential oil has a potent antimicrobial activity against some Gram (+) pathogenic bacteria and Botrytis cinerea fungi. The present studies confirm the use of this essential oil as anticancer agent. Further research is required to evaluate the practical values of therapeutic applications.

【 授权许可】

   
2011 Zarai et al; licensee BioMed Central Ltd.

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