Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms

Microbial Cell Factories

Review

Antônio Milton Vieira Gomes¹  Nádia Skorupa Parachin¹  Lúcio Rezende Queiroz¹  Lucas Silva Carvalho²  Juliana Davies de Oliveira³  Beatriz Simas Magalhães⁴ 

[1] Grupo de Engenharia Metabólica Aplicada a Bioprocessos, Instituto de Ciências Biológicas, Universidade de Brasília, CEP 70.790-900, Brasília, DF, Brazil;Integra Bioprocessos e Análises, Campus Universitário Darcy Ribeiro, Edifício CDT, Sala AT-36/37, CEP 70.904-970, Brasília, DF, Brazil;Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, CEP 70.790-160, Brasília, DF, Brazil;Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, CEP 70.790-160, Brasília, DF, Brazil;Integra Bioprocessos e Análises, Campus Universitário Darcy Ribeiro, Edifício CDT, Sala AT-36/37, CEP 70.904-970, Brasília, DF, Brazil;

关键词: Hyaluronic acid;    Hyaluron synthase;    Streptococcus zooepidemicus;    Bacillus subtillis;    Pichia pastoris;    Pasteurella multocida;    Escherichia coli;   

DOI  :  10.1186/s12934-016-0517-4

 received in 2016-02-24, accepted in 2016-06-22,  发布年份 2016

来源: Springer

【 摘 要 】

Hyaluronic acid, or HA, is a rigid and linear biopolymer belonging to the class of the glycosaminoglycans, and composed of repeating units of the monosaccharides glucuronic acid and N-acetylglucosamine. HA has multiple important functions in the human body, due to its properties such as bio-compatibility, lubricity and hydrophilicity, it is widely applied in the biomedical, food, health and cosmetic fields. The growing interest in this molecule has motivated the discovery of new ways of obtaining it. Traditionally, HA has been extracted from rooster comb-like animal tissues. However, due to legislation laws HA is now being produced by bacterial fermentation using Streptococcus zooepidemicus, a natural producer of HA, despite it being a pathogenic microorganism. With the expansion of new genetic engineering technologies, the use of organisms that are non-natural producers of HA has also made it possible to obtain such a polymer. Most of the published reviews have focused on HA formulation and its effects on different body tissues, whereas very few of them describe the microbial basis of HA production. Therefore, for the first time this review has compiled the molecular and genetic bases for natural HA production in microorganisms together with the main strategies employed for heterologous production of HA.

【 授权许可】

CC BY   
© The Author(s) 2016