MicroRNA profiling of rhesus macaque embryonic stem cells

BMC Genomics

Research Article

Xiechao He¹  Weizhi Ji¹  Qiang Wei²  Bing Su³  Zhenghua Sun⁴  Yanfeng Zhang⁴ 

[1] Department of Reproduction and Development, Kunming Institute of Zoology, Chinese Academy of Sciences, 650223, Kunming, China;Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, 650051, Kunming, China;Department of Reproduction and Development, Kunming Institute of Zoology, Chinese Academy of Sciences, 650223, Kunming, China;Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, 650051, Kunming, China;Graduate School of Chinese Academy of Sciences, 100049, Beijing, China;State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 650223, Kunming, China;Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, 650051, Kunming, China;State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 650223, Kunming, China;Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, 650051, Kunming, China;Graduate School of Chinese Academy of Sciences, 100049, Beijing, China;

关键词: Embryonic Stem Cell;    miRNA Expression;    Inner Cell Mass;    miRNA Precursor;    Human ESCs;   

DOI  :  10.1186/1471-2164-12-276

 received in 2010-12-30, accepted in 2011-05-31,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) play important roles in embryonic stem cell (ESC) self-renewal and pluripotency. Numerous studies have revealed human and mouse ESC miRNA profiles. As a model for human-related study, the rhesus macaque is ideal for delineating the regulatory mechanisms of miRNAs in ESCs. However, studies on rhesus macaque (r)ESCs are lacking due to limited rESC availability and a need for systematic analyses of fundamental rESC characteristics.ResultsWe established three rESC lines and profiled microRNA using Solexa sequencing resulting in 304 known and 66 novel miRNAs. MiRNA profiles were highly conserved between rESC lines and predicted target genes were significantly enriched in differentiation pathways. Further analysis of the miRNA-target network indicated that gene expression regulated by miRNAs was negatively correlated to their evolutionary rate in rESCs. Moreover, a cross-species comparison revealed an overall conservation of miRNA expression patterns between human, mouse and rhesus macaque ESCs. However, we identified three miRNA clusters (miR-467, the miRNA cluster in the imprinted Dlk1-Dio3 region and C19MC) that showed clear interspecies differences.ConclusionsrESCs share a unique miRNA set that may play critical roles in self-renewal and pluripotency. MiRNA expression patterns are generally conserved between species. However, species and/or lineage specific miRNA regulation changed during evolution.

【 授权许可】

CC BY   
© Sun et al; licensee BioMed Central Ltd. 2011