Direct targets of Klf5 transcription factor contribute to the maintenance of mouse embryonic stem cell undifferentiated state

BMC Biology

Research Article

Luca Cozzuto¹  Simona Ciriello¹  Tommaso Russo²  Lucio Pastore²  Silvia Parisi³  Dario Antonini³  Luigi Aloia³  Carolina Tarantino³  Fabiana Passaro⁴  Vincenzo De Simone⁴ 

[1] CEINGE Biotecnologie Avanzate, Via Gaetano Salvatore 482, 80145, Naples, Italy;CEINGE Biotecnologie Avanzate, Via Gaetano Salvatore 482, 80145, Naples, Italy;Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli "Federico II", Via Sergio Pansini 5, 80131, Naples, Italy;CEINGE Biotecnologie Avanzate, Via Gaetano Salvatore 482, 80145, Naples, Italy;European School of Molecular Medicine (SEMM), Via Gaetano Salvatore 482, 80145, Naples, Italy;Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli "Federico II", Via Sergio Pansini 5, 80131, Naples, Italy;

关键词: Embryonic Stem Cell;    Leukemia Inhibitory Factor;    Inner Cell Mass;    Mouse ESCs;    Klf5 Expression;   

DOI  :  10.1186/1741-7007-8-128

 received in 2010-09-10, accepted in 2010-09-27,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundA growing body of evidence has shown that Krüppel-like transcription factors play a crucial role in maintaining embryonic stem cell (ESC) pluripotency and in governing ESC fate decisions. Krüppel-like factor 5 (Klf5) appears to play a critical role in these processes, but detailed knowledge of the molecular mechanisms of this function is still not completely addressed.ResultsBy combining genome-wide chromatin immunoprecipitation and microarray analysis, we have identified 161 putative primary targets of Klf5 in ESCs. We address three main points: (1) the relevance of the pathways governed by Klf5, demonstrating that suppression or constitutive expression of single Klf5 targets robustly affect the ESC undifferentiated phenotype; (2) the specificity of Klf5 compared to factors belonging to the same family, demonstrating that many Klf5 targets are not regulated by Klf2 and Klf4; and (3) the specificity of Klf5 function in ESCs, demonstrated by the significant differences between Klf5 targets in ESCs compared to adult cells, such as keratinocytes.ConclusionsTaken together, these results, through the definition of a detailed list of Klf5 transcriptional targets in mouse ESCs, support the important and specific functional role of Klf5 in the maintenance of the undifferentiated ESC phenotype.See: http://www.biomedcental.com/1741-7007/8/125

【 授权许可】

CC BY   
© Parisi et al; licensee BioMed Central Ltd. 2010