Expression of natriuretic peptide receptor-A in esophageal squamous cell carcinomas and the relationship with tumor invasion and migration

World Journal of Surgical Oncology

Research

Haoqian Liu¹  Kai Sun²  Jia Zhang²  Jiansheng Wang²  Hui Cai²  Ya Yang²  Min Li²  Zhilong Zhao³ 

[1] Department of Surgical Oncology, Baoji Central hospital, Jiang Tan Road no. 8, 721000, Baoji, Shaanxi, China;Department of second Thoracic surgery, First Affiliated Hospital, Xi'an Jiaotong University, Yanta West Road no. 277, 710061, Xi'an, Shaanxi, China;Department of second Thoracic surgery, First Affiliated Hospital, Xi'an Jiaotong University, Yanta West Road no. 277, 710061, Xi'an, Shaanxi, China;Department of Surgical Oncology, Baoji Central hospital, Jiang Tan Road no. 8, 721000, Baoji, Shaanxi, China;

关键词: natriuretic peptide receptor-A;    NPRA;    esophageal squamous cell carcinoma;    ESCC;    MMP;    invasion;    migration;   

DOI  :  10.1186/1477-7819-12-154

 received in 2013-12-11, accepted in 2014-05-06,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe natriuretic peptide receptor-A (NPRA) has been investigated as a receptor of natriuretic peptides in the cardiovascular system. In this study, however, we analyze the expression status of NPRA and the relationship with tumor invasion in esophageal squamous cell carcinoma (ESCC) for the first time.MethodsWestern blots were used to examine the expression status of protein in human ESCC cell lines. Then, we used immunohistochemistry to detect the expression of NPRA in 45 ESCC specimens and 40 corresponding nontumor tissues. The clinical data were analyzed through statistical methods. Sh-RNA-NPRA was transfected into Eca109 cells to detect the relationship between NPRA and cell invasion through transwell assays.ResultsIn esophageal squamous cells, the expression of NPRA was strongly detected in the cytoplasm, while undetectable or very weak in the nucleus. The positive rates of NPRA in cancer tissues are significantly higher than that in nontumor tissues (P <0.05). Clinicopathological analyses revealed that increased NPRA expression correlated with differentiation and TNM stage (P <0.05), while it showed no statistically significant association with age, gender, and lymph node metastasis. In analysis of prognosis, we found that highly.Transwell assays showed that NPRA promoted Eca109 cell migration and invasion in vitro and may be involved in MMP2 and MMP9 activation.ConclusionsNPRA protein is highly expressed in ESCC tissues and could promote Eca109 cell migration and invasion in vitro.

【 授权许可】

Unknown   
© Zhao et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.