Coexpression analysis of lncRNAs and mRNAs identifies potential regulatory long noncoding RNAs involved in the inflammatory effects of lipopolysaccharide on bovine mammary epithelial cells

BMC Veterinary Research

Research

Xiaojing Xia¹  Lei Wang¹  Jianhe Hu¹  Zhanwei Teng¹  Jie Hou¹  Xiaobing Wei¹  Pengfei Ren¹  Mingcheng Liu²  Oksana Kasianenko³  Likun Cheng⁴ 

[1] College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, 453003, Xinxiang, PR China;College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, 453003, Xinxiang, PR China;Faculty of Veterinary Medicine, Sumy National Agrarian University, Sumy, Ukraine;Faculty of Veterinary Medicine, Sumy National Agrarian University, Sumy, Ukraine;Shandong Binzhou Animal Science and Veterinary Medicine Academy, 256600, Binzhou, PR China;

关键词: Mastitis;    lncRNA;    mRNA;    Mammary gland epithelial cells;    RNA-seq;   

DOI  :  10.1186/s12917-023-03780-4

 received in 2023-06-13, accepted in 2023-10-07,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe infection of bovine mammary glands by pathogenic microorganisms not only causes animal distress but also greatly limits the development of the dairy industry and animal husbandry. A deeper understanding of the host’s initial response to infection may increase the accuracy of selecting drug-resistant animals or facilitate the development of new preventive or therapeutic intervention strategies. In addition to their functions of milk synthesis and secretion, bovine mammary epithelial cells (BMECs) play an irreplaceable role in the innate immune response. To better understand this process, the current study identified differentially expressed long noncoding lncRNAs (DE lncRNAs) and mRNAs (DE mRNAs) in BMECs exposed to Escherichia coli lipopolysaccharide (LPS) and further explored the functions and interactions of these lncRNAs and mRNAs.ResultsIn this study, transcriptome analysis was performed by RNA sequencing (RNA-seq), and the functions of the DE mRNAs and DE lncRNAs were predicted by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Next, we constructed a modulation network to gain a deeper understanding of the interactions and roles of these lncRNAs and mRNAs in the context of LPS-induced inflammation. A total of 231 DE lncRNAs and 892 DE mRNAs were identified. Functional enrichment analysis revealed that pathways related to inflammation and the immune response were markedly enriched in the DE genes. In addition, research results have shown that cell death mechanisms, such as necroptosis and pyroptosis, may play key roles in LPS-induced inflammation.ConclusionsIn summary, the current study identified DE lncRNAs and mRNAs and predicted the signaling pathways and biological processes involved in the inflammatory response of BMECs that might become candidate therapeutic and prognostic targets for mastitis. This study also revealed several possible pathogenic mechanisms of mastitis.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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