Study of Helicobacter pylori genotype status in cows, sheep, goats and human beings

BMC Gastroenterology

Research Article

Hossein Dabiri¹  Hassan Momtaz²  Mohsen Gholami³  Negar Souod⁴ 

[1] Department of Medical Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran;Department of Microbiology, Faculty of Veterinary Medicine, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran;Graduated of Veterinary Medicine, Faculty of Veterinary Medicine, ShahreKord Branch, Islamic Azad University, ShahreKord, Iran;Young Researchers and Elite club, Central Tehran Branch, Islamic Azad University, P.O.Box: 13185-768, Tehran, Iran;

关键词: Helicobacter pylori;    Virulence genes;    Cow;    Sheep;    Goat;    Human being;   

DOI  :  10.1186/1471-230X-14-61

 received in 2013-08-11, accepted in 2014-03-21,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundHelicobacter pylori is one of the most controversial bacteria in the world causing diverse gastrointestinal diseases. The transmission way of this bacterium still remains unknown. The possibility of zoonotic transmission of H. pylori has been suggested, but is not proven in nonprimate reservoirs. In the current survey, we investigate the presence of H. pylori in cow, sheep and goat stomach, determine the bacterium virulence factors and finally compare the human H. pylori virulence factors and animals in order to examine whether H. pylori might be transmitted from these animals to human beings.MethodsThis cross- sectional study was performed on 800 gastric biopsy specimens of cows, sheep, goats and human beings. The PCR assays was performed to detection of H. pylori, vacA and cagA genes. The PCR products of Ruminant’s samples with positive H. pylori were subjected to DNA sequencing analysis. Statistical tests were applied for data analysis.ResultsOverall 6 (3%) cows, 32 (16%) sheep and 164 (82%) human beings specimens were confirmed to be H. pylori positive; however we were not able to detect this bacterium in all 200 goat samples. The vacA s1a/m1a was the predominant H. pylori genotype in all three kinds of studied population. There was 3.4–8.4% variability and 92.9-98.5% homology between sheep and human samples.ConclusionsConsidering the high sequence homology among DNA of H. pylori isolated from sheep and human, our data suggest that sheep may act as a reservoir for H. pylori and in the some extent share the ancestral host for the bacteria with human.

【 授权许可】

Unknown   
© Momtaz et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.