Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers

BMC Veterinary Research

Research Article

Laura Arroyo¹  Anna Marco-Ramell²  Anna Bassols³  Raquel Peña³  Raquel Pato³  Yolanda Saco³  Lorenzo Fraile⁴  Emøke Bendixen⁵ 

[1] Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Spain;Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Spain;Present Address: Departament de Nutrició, Ciències de l’Alimentació i Gastronomia, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, 08028, Barcelona, Spain;Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Spain;Servei de Bioquímica Clínica Veterinària, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Spain;Departament de Ciencia Animal, Universitat de Lleida, 25198, Lleida, Spain;Agrotecnio Center, Lleida, Spain;Department of Molecular Biology and Genetics, Aarhus University, 8000, Aarhus C, Denmark;

关键词: Acute phase proteins;    Biomarker;    Individual confinement;    Innate immunity;    Lipids;    Oxidative stress;    Pig;    Proteomics;    Stress;   

DOI  :  10.1186/s12917-016-0887-1

 received in 2015-08-25, accepted in 2016-11-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe objective assessment of animal stress and welfare requires proper laboratory biomarkers. In this work, we have analyzed the changes in serum composition in gilts after switching their housing, from pen to individual stalls, which is generally accepted to cause animal discomfort.ResultsBlood and saliva samples were collected a day before and up to four days after changing the housing system. Biochemical analyses showed adaptive changes in lipid and protein metabolism after the housing switch, whereas cortisol and muscular markers showed a large variability between animals. 2D-DIGE and iTRAQ proteomic approaches revealed variations in serum protein composition after changing housing and diet of gilts. Both techniques showed alterations in two main homeostatic mechanisms: the innate immune and redox systems. The acute phase proteins haptoglobin, apolipoprotein A-I and α1-antichymotrypsin 3, and the antioxidant enzyme peroxiredoxin 2 were found differentially expressed by 2D-DIGE. Other proteins related to the innate immune system, including lactotransferrin, protegrin 3 and galectin 1 were also identified by iTRAQ, as well as oxidative stress enzymes such as peroxiredoxin 2 and glutathione peroxidase 3. Proteomics also revealed the decrease of apolipoproteins, and the presence of intracellular proteins in serum, which may indicate physical injury to tissues.ConclusionsHousing of gilts in individual stalls and diet change increase lipid and protein catabolism, oxidative stress, activate the innate immune system and cause a certain degree of tissue damage. We propose that valuable assays for stress assessment in gilts may be based on a score composed by a combination of salivary cortisol, lipid metabolites, innate immunity and oxidative stress markers and intracellular proteins.

【 授权许可】

CC BY   
© The Author(s). 2016