Bioinformatic analysis of microRNA and mRNA Regulation in peripheral blood mononuclear cells of patients with chronic obstructive pulmonary disease

Respiratory Research

Research

Xiaoyan Qu¹  Dan Xu¹  Ying Chang¹  Weijia Wang¹  Chongbing Liao¹  Ying Li¹  Xiaojin Zhang¹  Xiaomin Dang²  Dong Shang²  Carolyn J. Baglole³ 

[1] Center for Translational Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology, Xi’an Jiaotong University, 710049, Xi’an, Shaanxi province, China;Department of Respiration, The First Affiliated Hospital, Xi’an Jiaotong University, 710061, Xi’an, Shaanxi province, China;Meakins-Christie Laboratories and Respiratory Division, Health Centre and Department of Medicine, McGill University, Montreal, QC, Canada;

关键词: miRNA;    COPD;    PBMC;    Microarray;    MicroRNA;   

DOI  :  10.1186/s12931-016-0486-5

 received in 2016-05-31, accepted in 2016-12-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundChronic obstructive pulmonary disease (COPD) is a progressive, irreversible chronic inflammatory disorder typified by increased recruitment of monocytes, lymphocytes and neutrophils. Because of this, as well as the convenience of peripheral blood nuclear cells (PBMCs) assessments, miRNA profiling of PBMCs has drawn increasing attention in recent years for various disease. Therefore, we analyzed miRNA and mRNA profiles to understand their regulatory network between COPD subjects versus smokers without airflow limitation.MethodsmiRNA and mRNA profiling of PBMCs from pooled 17 smokers and 14 COPD subjects was detected by high-throughput microarray. The expression of dysregulated miRNAs were validated by q-PCR. The miRNA targets in dysregulated mRNAs were predicted and the pathway enrichment was analyzed.ResultsmiRNA microarray showed that 8 miRNAs were up-regulated and 3 miRNAs were down-regulated in COPD subjects compared with smokers; the upregulation of miR-24-3p, miR-93-5p, miR-320a and miR-320b and the downregulation of miR-1273 g-3p were then validated. Bioinformatic analysis of regulatory network between miRNA and mRNA showed that NOD and TLR were the most enriched pathways. miR-24-3p was predicted to regulate IL-18, IL-1β, TNF, CCL3 and CCL4 and miR-93-5p to regulate IκBα.ConclusionsThe expression of miRNA and mRNA were dysregulated in PBMCs of COPD patients compared with smokers without airflow limitation. The regulation network between the dysregulated miRNA and mRNA may provide potential therapeutic targets for COPD.

【 授权许可】

CC BY   
© The Author(s). 2017